Frequency of micronuclei in hepatocytes following X and fast-neutron irradiations--an analysis by a linear-quadratic model.
Frequency of micronuclei in hepatocytes following X and fast-neutron irradiations--an analysis by a linear-quadratic model.
复制标题
X 射线和快中子辐照后肝细胞中微核的频率——线性二次模型分析。
作者：
K. Ono;Y. Nagata;K. Akuta;M. Abe;K. Ando;S. Koike
研究了微核试验用于研究肝细胞辐射反应的有用性。频率定义为微核总数与检查的肝细胞数量的比值。剂量-反应曲线在X射线照射后呈曲线型，在中子照射后呈直线型。通过线性二次模型（频率= aD + bD 2 + c）分析这些剂量-反应曲线。X线照射后a/B比值为3.03 ± 1.26戈伊。该值在其他人使用肝细胞克隆形成试验报告的α/β比值范围内。中子的a/B值为24.3 +/- 11.7戈伊，中子的最大相对生物学效应为6.30 +/- 2.53。由于微核试验简单、快速，它可能是一种很好的工具，在体内评价肝细胞的辐射反应。
The usefulness of the micronucleus assay for investigating the radiation response of hepatocytes was examined. The frequency was defined as the ratio of the total number of micronuclei to the number of hepatocytes examined. The dose-response curves were curvilinear after X rays and linear after neutrons. These dose-response curves were analyzed by a linear-quadratic model, frequency = aD + bD2 + c. The a/b ratio was 3.03 +/- 1.26 Gy following X irradiation. This value is within the range of the alpha/beta ratios reported by others using the clonogenic assay of hepatocytes. While the a/b value for neutrons was 24.3 +/- 11.7 Gy, the maximum relative biological effectiveness of neutrons was 6.30 +/- 2.53. Since the micronucleus assay is simple and rapid, it may be a good tool for evaluating the radiation response of hepatocytes in vivo.
用于确定暴露于电离辐射的实质肝细胞的克隆存活的移植系统。
影响因子：
11.2
作者：
Jirtle,RL;Michalopoulos,G;McLain,JR;Crowley,J
通讯作者：
Crowley,J