SERODIAGNOSIS OF HUMAN LEPTOSPIROSIS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)

SERODIAGNOSIS OF HUMAN LEPTOSPIROSIS BY ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)
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DOI：
10.1016/s0172-5599(80)80045-9
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发表时间：
1980-01-01
期刊：
ZENTRALBLATT FUR BAKTERIOLOGIE MIKROBIOLOGIE UND HYGIENE SERIES A-MEDICAL MICROBIOLOGY INFECTIOUS DISEASES VIROLOGY PARASITOLOGY
影响因子：
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通讯作者：
SCHOONE, GJ
SCHOONE, GJ
中科院分区：
其他
文献类型：
--
作者：
TERPSTRA, WJ;LIGTHART, GS;SCHOONE, GJ

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本文介绍了一种用于钩端螺旋体病血清学诊断的酶联免疫吸附试验（ELISA）。使用从单一黄疸出血型钩端螺旋体菌株（Wijnberg）的加热培养物制备的抗原，ELISA是一种属特异性检测。显微镜凝集试验（MAT）作为参考。96例钩体病患者血清的ELISA和MAT结果符合率为95%。在217例对照中发现1例假阳性。ELISA是敏感的，特异性的，并且相对容易执行。
An enzyme-linked-immunosorbent-assay (ELISA) is described for the serodiagnosis of leptospirosis. Using an antigen prepared from a heated culture of a single Leptospira icterohaemorrhagiae strain (Wijnberg) the ELISA is a genus-specific test. The microscopic agglutination test (MAT) served as a reference. ELISA and MAT results agreed in 95% of the sera from 96 leptospirosis patients. One false positive was found in 217 controls. The ELISA is sensitive, specific and relatively easy to perform.