Calibration-Free Electrochemical Biosensors Supporting Accurate Molecular Measurements Directly in Undiluted Whole Blood.
Calibration-Free Electrochemical Biosensors Supporting Accurate Molecular Measurements Directly in Undiluted Whole Blood.
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DOI：
10.1021/jacs.7b05412
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发表时间：
2017-08-16
影响因子：
15
通讯作者：
Plaxco KW
中科院分区：
文献类型：
--
作者：
Li H;Dauphin-Ducharme P;Ortega G;Plaxco KW
校准以校正传感器到传感器制造变化和传感器漂移的需要已被证明是生物传感器广泛使用中的重大障碍。例如，为了保持临床相关（本应用为±20%）的准确度，商用连续葡萄糖监测仪需要每天多次重新校准，这降低了便利性并增加了用户错误的可能性。然而，在这里，我们展示了一个“双频”的方法，用于实现校准的自由操作的电化学生物传感器，产生一个输出，通过使用方波伏安法监测绑定诱导的电子转移动力学的变化。具体而言，我们使用它们响应的方波频率依赖性来产生比率信号，即在响应和非（或低）响应方波频率下收集的峰值电流的比率，这在很大程度上对漂移和传感器到传感器的制造变化不敏感。使用基于电化学适体（E-AB）的生物传感器作为我们的测试平台，我们展示了传感器对多种药物的准确和精确操作，在高达2个数量级的动态范围内，其目标测量的准确度优于20%，而无需校准每个单独的传感器。
The need to calibrate to correct for sensor-to-sensor fabrication variation and sensor drift has proven a significant hurdle in the widespread use of biosensors. To maintain clinically relevant (±20% for this application) accuracy, for example, commercial continuous glucose monitors require recalibration several times a day, decreasing convenience and increasing the chance of user errors. Here, however, we demonstrate a “dual-frequency” approach for achieving the calibration-free operation of electrochemical biosensors that generate an output by using square-wave voltammetry to monitor binding-induced changes in electron transfer kinetics. Specifically, we use the square-wave frequency dependence of their response to produce a ratiometric signal, the ratio of peak currents collected at responsive and non- (or low) responsive square-wave frequencies, which is largely insensitive to drift and sensor-to-sensor fabrication variations. Using electrochemical aptamer-based (E-AB) biosensors as our test bed, we demonstrate the accurate and precise operation of sensors against multiple drugs, achieving accuracy in the measurement of their targets of within better than 20% across dynamic ranges of up to 2 orders of magnitude without the need to calibrate each individual sensor.
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DOI：
10.1039/c3an01079a
发表时间：
2013-10-07
期刊：
The Analyst
影响因子：
--
作者：
Bonham AJ;Paden NG;Ricci F;Plaxco KW
通讯作者：
Plaxco KW
DOI：
10.1021/la202882k
发表时间：
2012-01-10
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Feld DJ;Hsu HT;Eckermann AL;Meade TJ
通讯作者：
Meade TJ
DOI：
10.2337/diaspect.27.3.174
发表时间：
2014-08
期刊：
Diabetes spectrum : a publication of the American Diabetes Association
影响因子：
--
作者：
Klonoff DC
通讯作者：
Klonoff DC
影响因子：
5.1
作者：
Kilo, Charles, Sr.;Dickey, William T., Jr.;Parker, Donald R.
通讯作者：
Parker, Donald R.
影响因子：
62.1
作者：
Genereux JC;Barton JK
通讯作者：
Barton JK