Applications for next-generation sequencing in fish ecotoxicogenomics.
Applications for next-generation sequencing in fish ecotoxicogenomics.
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DOI：
10.3389/fgene.2012.00062
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发表时间：
2012
影响因子：
3.7
通讯作者：
Denslow ND
中科院分区：
文献类型：
--
作者：
Mehinto AC;Martyniuk CJ;Spade DJ;Denslow ND
在分子医学中广泛应用的下一代测序和全局基因表达分析新技术正越来越多地应用于鱼类生物学领域。这为解决以前由于缺乏生态相关物种的分子信息而不能考虑的研究领域提供了新的方向。在过去的十年里，NGS的成本显著下降，使得使用非模式鱼类物种来调查新出现的环境问题成为可能。NGS技术使研究人员能够在短时间内获得大量原始数据。生物信息学在组装序列和注释基因方面也有了显著的进步，从而促进了这些大型数据集的管理。DNA测序和生物信息学的结合提高了我们设计定制微阵列以及研究各种生物的基因组和转录组的能力。尽管在鱼类研究中使用这些技术取得了令人振奋的结果，但NGS技术目前在生态毒物基因组学中未得到充分利用，而且很少有研究采用这些方法。应该解决这些问题，以便在生态毒理学研究中充分发挥NGS的潜力，并扩大我们对非模式生物生物学的理解。
The new technologies for next-generation sequencing (NGS) and global gene expression analyses that are widely used in molecular medicine are increasingly applied to the field of fish biology. This has facilitated new directions to address research areas that could not be previously considered due to the lack of molecular information for ecologically relevant species. Over the past decade, the cost of NGS has decreased significantly, making it possible to use non-model fish species to investigate emerging environmental issues. NGS technologies have permitted researchers to obtain large amounts of raw data in short periods of time. There have also been significant improvements in bioinformatics to assemble the sequences and annotate the genes, thus facilitating the management of these large datasets.The combination of DNA sequencing and bioinformatics has improved our abilities to design custom microarrays and study the genome and transcriptome of a wide variety of organisms. Despite the promising results obtained using these techniques in fish studies, NGS technologies are currently underused in ecotoxicogenomics and few studies have employed these methods. These issues should be addressed in order to exploit the full potential of NGS in ecotoxicological studies and expand our understanding of the biology of non-model organisms.