Propagation and Characterization of Influenza Virus Stocks That Lack High Levels of Defective Viral Genomes and Hemagglutinin Mutations.
Propagation and Characterization of Influenza Virus Stocks That Lack High Levels of Defective Viral Genomes and Hemagglutinin Mutations.
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DOI：
10.3389/fmicb.2016.00326
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发表时间：
2016
影响因子：
5.2
通讯作者：
López CB
中科院分区：
文献类型：
--
作者：
Xue J;Chambers BS;Hensley SE;López CB
流感病毒感染每年造成25万多人死亡。流感病毒的分离、繁殖和表征方案对于完成可重复的基础研究和产生疫苗种子储备至关重要。最近已经报道了用于分离和鉴定流感病毒的详细方案（Rumefeld等人，）。然而，很少有标准化的方案集中在病毒分离株的繁殖和表征，因此，在不同实验室的不同条件下繁殖的病毒通常具有不同的体外和体内特征。在这里，我们专注于甲型流感病毒在实验室中的繁殖和表征，同时考虑到病毒原液的整体质量和组成，以实现病毒产量、毒力和免疫刺激活性的一致性。
Influenza virus infections are responsible for more than 250,000 deaths annually. Influenza virus isolation, propagation, and characterization protocols are critical for completing reproducible basic research studies and for generating vaccine seed stocks. Detailed protocols for the isolation and identification of influenza virus have been recently reported (Eisfeld et al.,). However, there are few standardized protocols focused on the propagation and characterization of viral isolates, and as a result, viruses propagated in different conditions in different laboratories often have distinct in vitro and in vivo characteristics. Here, we focus on influenza A virus propagation and characterization in the laboratory taking into consideration the overall quality and composition of the virus stock to achieve consistency in virus yield, virulence, and immunostimulatory activity.