Diagnosis of malaria:: a review of alternatives to conventional microscopy
Diagnosis of malaria:: a review of alternatives to conventional microscopy
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DOI：
10.1046/j.1365-2257.1999.00220.x
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
Hänscheid, T
中科院分区：
文献类型：
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作者：
Hänscheid, T
疟疾在全世界，包括疟疾主要为输入性疾病的国家，造成严重的发病和死亡。在发展中国家，资源稀缺导致诊断程序不足。在富裕国家，对疟疾不熟悉可能导致临床和实验室误诊。Giemsa染色厚膜和薄膜的显微镜检查仍然是目前的诊断标准。虽然它具有良好的灵敏度，并允许物种识别和寄生虫计数，但它很耗时，需要显微镜专业知识和设备维护。荧光染色显微镜检查（QBC（R））、HRP 2和pLDH试纸抗原检测（Parasight（R）-F、ICT Malaria Pf（R）、OptiMAL（R））、聚合酶链反应分析和一些自动血细胞分析仪提供了新的方法，本文对其进行了综述，重点是临床相关性及其补充传统显微镜检查的潜力，特别是在疟疾输入国家。
Malaria causes significant morbidity and mortality worldwide, including countries with mainly imported malaria. In developing nations, scarce resources lead to inadequate diagnostic procedures. In affluent countries, poor familiarity with malaria may cause clinical and laboratory misdiagnosis. Microscopy of Giemsa-stained thick and thin films remains the current standard for diagnosis. Although it has good sensitivity and allows species identification and parasite counts, it is time consuming, requires microscopical expertise and maintenance of equipment. Microscopy with fluorescent stains (QBC (R)), dipstick antigen detection of HRP2 and pLDH (Parasight (R)-F, ICT Malaria Pf (R), OptiMAL (R)), polymerase chain reaction assays and some automated blood cell analysers offer new approaches and are reviewed here, with emphasis on clinical relevance and their potential to complement conventional microscopy, especially in countries with imported malaria.