Selective production of cis-9,trans-11 isomer of conjugated linoleic acid from trans-vaccenic acid methyl ester by Delacroixia coronata
Selective production of cis-9,trans-11 isomer of conjugated linoleic acid from trans-vaccenic acid methyl ester by Delacroixia coronata
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DOI：
10.1111/j.1365-2672.2008.04139.x
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发表时间：
2009-05-01
影响因子：
4
通讯作者：
Shimizu, S.
中科院分区：
文献类型：
--
作者：
Ando, A.;Ogawa, J.;Shimizu, S.
以反式十八烯酸（t-VA，trans-11-octadecenoic acid）为原料，通过微生物脂肪酸Delta 9-去饱和反应，异构体选择性高效生产顺式-9，反式-11-十八碳二烯酸（cis-9，trans-11-octadecadienoic acid，CLA）。选择Delacroixia coronata IFO 8586作为来自t-VA的CLA的有效生产者。有效的CLA生产过程中观察到的培养基中补充的甲基酯的t-VA（t-VAME）。在最佳条件下，以33.3mg/ml t-VAME为底物，D. coronata IFO 8586在含糊精（5.0%）、胰蛋白胨（2.0%）和硫脲（0.83 μ mol ml（-1））的培养基中培养7天后，该菌株选择性地产生共轭亚油酸的顺式9，反式11异构体（占共轭亚油酸总量的98%），同时还产生少量的反式9，反式11异构体（占共轭亚油酸总量的2%），主要以三酰甘油的形式存在（占共轭亚油酸总量的69%）。本研究成功地建立了cis-9，trans-11-CLA异构体选择性生物合成工艺。该工艺有利于扩大共轭亚油酸在医药和保健品方面的应用。
Bio-process development for isomer selective and efficient production of cis-9,trans-11-octadecadienoic acid (CLA) from trans-vaccenic acid (t-VA, trans-11-octadecenoic acid) through microbial fatty acid Delta 9-desaturation reaction.A total of 550 strains of fungi and yeasts were screened for CLA production from t-VA through Delta 9 desaturation. Delacroixia coronata IFO 8586 was selected as a potent producer of CLA from t-VA. Efficient CLA production was observed during cultivation in medium supplemented with the methyl ester of t-VA (t-VAME). Under the optimal conditions with 33.3 mg ml(-1) of t-VAME as substrate, 10.5 mg ml(-1) CLA was produced by D. coronata IFO 8586 after 7 days of cultivation in the medium containing dextrin (5.0%), tryptone (2.0%) and thiourea (0.83 mu mol ml(-1)). The strain produced the cis-9,trans-11 isomer of CLA selectively (98% of total CLA), with a small amount of the trans-9,trans-11 isomer (2% of total CLA), mainly in the form of triacylglycerols (69% of total CLA).A practical bio-process for selective production of cis-9,trans-11 isomer of CLA using filamentous fungus D. coronata IFO 8586 was successfully established.Isomer selective bio-process for the practical production of cis-9,trans-11-CLA was first established. The process is benefitable for expanding the application of CLA for medicinal and nutraceutical purposes.