Transcript level responses of Plasmodium falciparum to antimycin A.
Transcript level responses of Plasmodium falciparum to antimycin A.
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DOI：
10.1016/j.protis.2012.01.003
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发表时间：
2012-09
期刊：
影响因子：
2.5
通讯作者：
Howe CJ
中科院分区：
文献类型：
--
作者：
Tarr SJ;Nisbet RE;Howe CJ
线粒体电子传递链对疟原虫是必不可少的，也是抗疟药阿托伐醌的靶点。疟原虫属的线粒体基因组是已知的最简化的，并且大多数线粒体蛋白质在细胞核中编码并在死亡后输入到线粒体中。许多生物体在线粒体和细胞核之间具有信号传导途径，以调节细胞核编码的细胞靶向蛋白的表达，例如响应于线粒体功能障碍。我们已经研究了同步恶性疟原虫处理LD 50浓度的复合物III抑制剂抗霉素A的转录谱，以调查是否存在这样的途径在寄生虫。基因表达出现了广泛的扰动。差异表达基因的转录本编码蛋白参与入侵，胁迫反应，核苷酸生物合成和呼吸富集。有些影响是由于药物处理的寄生虫的基因表达阶段延迟。然而，我们的数据表明，线粒体应激反应基因和参与嘧啶生物合成的基因的调节，这意味着存在从线粒体到细胞核的信号通路。
The mitochondrial electron transport chain is essential to Plasmodium and is the target of the antimalarial drug atovaquone. The mitochondrial genomes of Plasmodium sp. are the most reduced known, and the majority of mitochondrial proteins are encoded in the nucleus and imported into the mitochondrion post-translationally. Many organisms have signalling pathways between the mitochondria and the nucleus to regulate the expression of nuclear-encoded mitochondrially-targeted proteins, for example in response to mitochondrial dysfunction. We have studied the transcript profiles of synchronous Plasmodium falciparum treated with an LD50 concentration of the complex III inhibitor antimycin A, to investigate whether such pathways exist in the parasite. There was a broad perturbation of gene expression. The differentially expressed genes were enriched for transcripts encoding proteins involved in invasion, stress response, nucleotide biosynthesis and respiration. Some effects were attributable to a delay in the gene expression phase of drug-treated parasites. However, our data indicated regulation of mitochondrial stress response genes and genes involved in pyrimidine biosynthesis, implying the existence of a signalling pathway from the mitochondrion to the nucleus.
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DOI：
10.1042/bj20081386
发表时间：
2009-01-01
期刊：
The Biochemical journal
影响因子：
--
作者：
Murphy MP
通讯作者：
Murphy MP
影响因子：
6.7
作者：
Ganesan K;Ponmee N;Jiang L;Fowble JW;White J;Kamchonwongpaisan S;Yuthavong Y;Wilairat P;Rathod PK
通讯作者：
Rathod PK
影响因子：
6.7
作者：
Campbell TL;De Silva EK;Olszewski KL;Elemento O;Llinás M
通讯作者：
Llinás M
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
14.9
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.