Nonphotochemical quenching of chlorophyll fluorescence in Chlamydomonas reinhardtii
Nonphotochemical quenching of chlorophyll fluorescence in Chlamydomonas reinhardtii
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DOI：
10.1021/bi0521588
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发表时间：
2006-02-07
期刊：
影响因子：
2.9
通讯作者：
Wollman, FA
中科院分区：
文献类型：
--
作者：
Finazzi, G;Johnson, GN;Wollman, FA
与植物不同，莱茵衣藻在光照下产生非光化学猝灭的能力有限。这种有限的猝灭大部分是由于状态转变而不是 Delta pH 驱动的高能状态猝灭 (qE)。后者只有在细胞进行光合作用的能力受损时才能观察到，要么通过将温度降低到接近 0 摄氏度，要么在缺乏 RubisCO 活性的突变体中。衣藻中 qE 水平较低的两个主要特征已被确定。一方面，光照时产生的电化学质子梯度显然不足以促进荧光猝灭。另一方面，与植物相比，这种藻类将 Delta pH 的存在转化为猝灭响应的能力也本质上降低了。讨论了导致这些差异的可能机制。
Unlike plants, Chlamydomonas reinhardtii shows a restricted ability to develop nonphotochemical quenching upon illumination. Most of this limited quenching is due to state transitions instead of Delta pH-driven high-energy state quenching, qE. The latter could only be observed when the ability of the cells to perform photosynthesis was impaired, either by lowering temperature to similar to 0 degrees C or in mutants lacking RubisCO activity. Two main features were identified that account for the low level of qE in Chlamydomonas. On one hand, the electrochemical proton gradient generated upon illumination is apparently not sufficient to promote fluorescence quenching. On the other hand, the capacity to transduce the presence of a Delta pH into a quenching response is also intrinsically decreased in this alga, when compared to plants. The possible mechanism leading to these differences is discussed.