Experimental cryptosporidiosis in laboratory mice
Experimental cryptosporidiosis in laboratory mice
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实验室小鼠的实验性隐孢子虫病
作者：
D. Sherwood;K. Angus;Snodgrass Dr;S. Tzipori
8个品系的实验室小鼠在1至4日龄时对隐孢子虫属的亚临床感染敏感，但在21日龄或更大时只能建立一过性感染。对21天大的小鼠进行免疫抑制并没有使它们更容易感染。通过在1至4日龄小鼠中滴定，研究了隐孢子虫属的实验室储存条件。用各种冷冻保护剂冷冻储存是不成功的，但在4 ℃的磷酸盐缓冲盐水或2.5%重铬酸钾中储存4至6个月是可能的。
Eight strains of laboratory mice were susceptible to subclinical infections with Cryptosporidium sp. at 1 to 4 days of age, but only a transient infection could be established at 21 days of age or older. Immunosuppression of 21-day-old mice failed to render them more susceptible to infection. Laboratory storage conditions for Cryptosporidium sp. were investigated by titration in 1- to 4-day-old mice. Storage by freezing with a variety of cryoprotectants was unsuccessful, but storage at 4 degrees C in phosphate-buffered saline or 2.5% potassium dichromate was possible for 4 to 6 months.