Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis
Temperature-dependent variations and intraspecies diversity of the structure of the lipopolysaccharide of Yersinia pestis
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DOI：
10.1021/bi048430f
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发表时间：
2005-02-08
期刊：
影响因子：
2.9
通讯作者：
Anisimov, AP
中科院分区：
文献类型：
--
作者：
Knirel, YA;Lindner, B;Anisimov, AP
鼠疫耶尔森氏菌于 20 世纪初在整个美洲传播，在该地区主要以单一克隆形式出现。然而，在欧亚大陆和非洲部分地区，鼠疫耶尔森氏菌菌株之间存在显着的多样性，可分为不同的生物变型 (bv.) 和/或亚种 (ssp.)，其中 bv. 和/或亚种 (ssp.)侧柏/亚种。鼠疫菌与美国克隆鼠疫菌关系最为密切。为了确定这些不同鼠疫耶尔森氏菌分离株的相关性的一方面，确定了鼠疫耶尔森氏菌的四种野生型和一种LPS突变型欧亚/非洲菌株的脂多糖（LPS）结构，评估了在哺乳动物（37℃）或跳蚤（25℃）温度下生长对核心寡糖和脂质A的结构和组成的影响。在鼠疫菌中，单个主要核心糖型在 37°C 下合成，而多核心寡糖糖型在 25°C 下产生。结构差异主要发生在末端单糖中。在 37°C 时仅产生四酰基脂质 A，而在 25°C 时则产生额外的五酰基和六酰基脂质 A 结构。脂质 A 中的 4-氨基-4-脱氧阿拉伯糖水平随着生长温度的降低或在多粘菌素 B 存在下培养细菌而增加。在高加索地区，LPS核心缺乏D-甘油-D-甘露庚糖，并且4-氨基-4-脱氧阿拉伯糖的含量不依赖于生长温度，而脂质A的酰化程度和寡糖核心的结构则依赖于温度。自发的深层粗糙 LPS 突变菌株仅具有二糖核心和略有变异的脂质 A。鼠疫耶尔森氏菌 LPS 结构的多样性和差异表明这些变异对该生物体的发病机制有重要贡献，可能与鼠疫耶尔森氏菌的先天和后天免疫识别以及检测、分类、控制和应对鼠疫耶尔森氏菌感染的流行病学手段有关。
Yersinia pestis spread throughout the Americas in the early 20th century, and it occurs predominantly as a single clone within this part of the world. However, within Eurasia and parts of Africa there is significant diversity among Y. pestis strains, which can be classified into different biovars (bv.) and/or subspecies (ssp.), with bv. orientalis/ssp. pestis most closely related to the American clone. To determine one aspect of the relatedness of these different Y. pestis isolates, the structure of the lipopolysaccharide (LPS) of four wild-type and one LPS-mutant Eurasian/African strains of Y. pestis was determined, evaluating effects of growth at mammalian (37 degreesC) or flea (25 degreesC) temperatures on the structure and composition of the core oligosaccharide and lipid A. In the wild-type clones of ssp. pestis, a single major core glycoform was synthesized at 37 degreesC whereas multiple core oligosaccharide glycoforms were produced at 25 T. Structural differences occurred primarily in the terminal monosaccharides. Only tetraacyl lipid A was made at 37 'C, whereas at 25 'C additional pentaacyl and hexaacyl lipid A structures were produced. 4-Amino-4-deoxyarabinose levels in lipid A increased with lower growth temperatures or when bacteria were cultured in the presence of polymyxin B. In Y. pestis ssp. caucasica, the LPS core lacked D-glycero-D-manno-heptose and the content of 4-amino-4-deoxyarabinose showed no dependence on growth temperature, whereas the degree of acylation of the lipid A and the structure of the oligosaccharide core were temperature dependent. A spontaneous deep-rough LPS mutant strain possessed only a disaccharide core and a slightly variant lipid A. The diversity and differences in the structure of the Y pestis LPS suggest important contributions of these variations to the pathogenesis of this organism, potentially related to innate and acquired immune recognition of Y. pestis and epidemiologic means to detect, classify, control and respond to Y. pestis infections.