Evidence for a major route for zinc uptake in human red blood cells: [ZN(HCO3)2CL]−influx through the [CI−/HCO3−] anion exchanger
Evidence for a major route for zinc uptake in human red blood cells: [ZN(HCO3)2CL]−influx through the [CI−/HCO3−] anion exchanger
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人类红细胞吸收锌的主要途径的证据：[ZN(HCO3)2CL] - 通过 [CI - / HCO3 - ] 阴离子交换剂流入
DOI：
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发表时间：
1989
影响因子：
5.6
通讯作者：
R. Garay
中科院分区：
文献类型：
--
作者：
José Octavio Alda Torrubia;R. Garay
用原子吸收法测定了人红细胞对Zn ~（2+）的初始摄取速率。DIDS抑制了一个非常重要的Zn 2+摄取部分，IC 50 = 0.3（μ M）（呋塞米和布美他尼的IC 50分别为200和500 μ M）。DIDS敏感的Zn 2+吸收表现出以下特性：（1）它需要同时存在外部HCO 3 −和Cl−。(2)在含Cl−的介质中，它受到外部HCO 3 −的强烈刺激，遵循S形（S形）和饱和函数，该函数由Hanes方程拟合，n = 2，表观解离常数（对于外部HCO 3 −）为5.3 ± 0.9 mM（四个实验的平均值± SD）。在饱和HCO 3 −浓度下，Zn 2+吸收的最大速率为50.7 ± 4.8 mmol（升细胞x h）−1。(3)在含HCO 3 −的培养基中，它受到外部Cl−的强烈刺激，遵循Michaelis方程，表观解离常数为88 ± 11 mM（三个实验的平均值± SD）。4)生理浓度的磷酸盐可抑制Bicycle刺激的Zn 2+摄取（硫酸盐的抑制作用远低于磷酸盐）。动力学分析的数据强烈表明，锌是由一价阴离子络合物形式的阴离子载体：[Zn（HCO 3）2Cl]−。
The initial rate of Zn2+ uptake in human red cells was measured by atomic absorption. A very important fraction of Zn2+ uptake was inhibited by DIDS with IC50 = 0.3 (μ)M (and by furosemide and bumetanide with IC50 of 200 and 500 μ M, respectively). DIDS–sensitive Zn2+ uptake exhibited the following properties: (1) It required the simultaneous presence of both external HCO3− and Cl−. (2) In Cl− containing media, it was strongly stimulated by external HCO3− following a sigmoidal (S–shaped) and saturable function, which was fitted by a Hanes equation, with n = 2 and an apparent dissociation constant (for external HCO3−) of 5.3 ± 0.9 mM (mean ± SD of four experiments). The maximal rate of Zn2+ uptake at saturating HCO3− concentrations was 50.7 ± 4.8 mmol (liter cells x h)−1. (3) In HCO3− containing media, it was strongly stimulated by external Cl− following a Michaelis‐like equation with an apparent dissociation constant (for external Cl− of 88 ± 11 mM (mean ± SD of three experiments). 4) Bicarbonate‐stimulated Zn2+ uptake was inhibited by physiological concentrations of phosphate (sulfate was a much less potent inhibitor than phosphate). A kinetic analysis of the data strongly suggested that zinc was transported by the anion carrier in the form of the monovalent anion complex: [Zn(HCO3)2Cl]−.
红细胞中阴离子转运的动力学和机制。
影响因子：
18.2
作者：
Jennings,ML
通讯作者：
Jennings,ML