Spatial and temporal control of angiogenesis and arterialization using focal applications of VEGF164 and Ang-1
Spatial and temporal control of angiogenesis and arterialization using focal applications of VEGF164 and Ang-1
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DOI：
10.1152/ajpheart.00833.2003
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发表时间：
2004-03-01
影响因子：
4.8
通讯作者：
Skalak, TC
中科院分区：
文献类型：
--
作者：
Peirce, SM;Price, RJ;Skalak, TC
微血管网络经历模式变化，决定和反映组织重塑过程中的功能适应。网络架构的变化是复杂和集成的信号事件的结果。为了了解两种生长因子信号如何相互作用以刺激血管生成和动脉化，我们通过局部递送外源性生长因子的组合在体内设计了空间定向的微血管模式变化。我们将含有重组血管内皮生长因子-164 （VEGF(164)）和重组血管生成素-1* (Ang-1*)的微输送珠植入完全麻醉的雄性Fischer 344大鼠背部皮下组织，并通过活体显微镜、结构指标和免疫组织化学相结合来量化血管模式的变化。血管内皮生长因子（VEGF）的局灶性传递引起血管直径的总数和密度的空间定向增加
Microvascular networks undergo patterning changes that determine and reflect functional adaptations during tissue remodeling. Alterations in network architectures are a result of complex and integrated signaling events. To understand how two growth factor signals interact to stimulate angiogenesis and arterialization, we engineered spatially directed microvascular pattern changes in vivo by using combinations of focally delivered exogenous growth factors. We implanted microdelivery beads containing recombinant vascular endothelial growth factor-164 (VEGF(164)) and recombinant angiopoietin-1* (Ang-1*) into the dorsal subcutaneous tissue of fully anesthetized male Fischer 344 rats implanted with backpack window chambers, and we quantified vascular patterning changes by using intravital microscopy, a combination of architectural metrics, and immunohistochemistry. Focal delivery of VEGF(164) caused spatially directed increases in both the total number and the density of vessels with diameters