Analysis of in situ methylated microbial fatty acid constituents by curie-point pyrolysis—gas chromatography—mass spectrometry
Analysis of in situ methylated microbial fatty acid constituents by curie-point pyrolysis—gas chromatography—mass spectrometry
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居里点热解-气相色谱-质谱法原位甲基化微生物脂肪酸成分分析
DOI：
10.1016/s0021-9673(00)96315-5
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发表时间：
1989
影响因子：
4.1
通讯作者：
W. Bertsch
中科院分区：
文献类型：
--
作者：
G. Holzer;T. F. Bourne;W. Bertsch
利用新研制的居里点裂解仪分析微生物脂肪酸组成。热解在铁磁线上进行，铁磁线通过可伸缩的Pyrex玻璃管装载到气相色谱注射器-热解器中。游离脂肪酸和甘油结合脂肪酸与氢氧化三甲基苯胺原位甲基化得到脂肪酸甲酯。该方法成功地应用于整个微生物。从全细胞热解和相应生物体的脂质提取物的脂肪酸甲酯谱之间有很好的一致性。
A newly developed Curie-point pyrolyzer has been used to analyze microbial fatty acid constituents. The pyrolysis was carried out on ferromagnetic wires, which were loaded into the gas chromatographic injector—pyrolyzer via a retractable Pyrex glass tube. Fatty acid methyl esters were obtained byin situmethylation of the free and glycerol bound fatty acids with trimethylanilinium hydroxide. The procedure was applied sucessfully to whole microorganisms. There was good agreement between the fatty acid methyl ester profiles from the whole-cell pyrolysis and from the lipid extracts of the corresponding organisms.