Photodynamic treatment of endodontic polymicrobial infection in vitro
Photodynamic treatment of endodontic polymicrobial infection in vitro
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DOI：
10.1016/j.joen.2008.03.011
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发表时间：
2008-06-01
影响因子：
4.2
通讯作者：
Soukos, Nikolaos S.
中科院分区：
文献类型：
--
作者：
Fimple, Jacob Lee;Fontana, Carla Raquel;Soukos, Nikolaos S.
在体外实验感染的人拔牙根管中，研究了亚甲基蓝对包括以色列放线菌、核梭菌亚种、牙龈卟啉单胞菌和中间普雷沃菌在内的多种根管生物膜的光动力学影响。采用DNA探针检测根管内4种试验微生物。扫描电镜显示治疗前根管内存在生物膜。根管系统用亚甲基蓝（25 μ g/mL）孵育10分钟，然后暴露于665 nm、能量影响为30 J/cm(2)的红光下。光从二极管激光器通过直径250 μ m的聚甲基丙烯酸甲酯光纤传输，该光纤均匀分布在360度以上。光动力疗法（PDT）使菌落形成单位计数减少了80%。我们得出结论，当PDT参数优化时，PDT可以作为标准根管抗菌治疗的有效辅助。
We investigated the photodynamic effects of methylene blue on multispecies root canal biofilms comprising Actinomyces israelii, Fusobacterium nucleatum subspecies nucleatum, Porphyromonas gingivalis, and Prevotella intermedia in experimentally infected root canals of extracted human teeth in vitro. The 4 test microorganisms were detected in root canals by using DNA probes. Scanning electron microscopy showed the presence of biofilms in root canals before therapy. Root canal systems were incubated with methylene blue (25 mu g/mL) for 10 minutes followed by exposure to red light at 665 nm with an energy fluence of 30 J/cm(2). Light was delivered from a diode laser via a 250-mu m diameter polymethyl methacrylate optical fiber that uniformly distributed light over 360 degrees. Photodynamic therapy (PDT) achieved Lip to 80% reduction of colony-forming unit counts. We concluded that PDT can be an effective adjunct to standard endodontic antimicrobial treatment when the PDT parameters are optimized.