Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with Abscisic Acid Content under Drought Stress
Nucleotide Variation in the NCED3 Region of Arabidopsis thaliana and its Association Study with Abscisic Acid Content under Drought Stress
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DOI：
10.1111/j.1744-7909.2008.00786.x
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发表时间：
2009-02-01
影响因子：
11.4
通讯作者：
Huang, Cong-Lin
中科院分区：
文献类型：
--
作者：
Hao, Gang-Ping;Zhang, Xiu-Hai;Huang, Cong-Lin
耐旱性是一个综合性的数量性状，目前正在分子遗传水平上得到进一步的研究。脱落酸（阿坝）是干旱诱导的主要激素，它调节许多干旱相关基因的表达。9-顺式环氧类胡萝卜素双加氧酶（NCED 3）是阿坝生物合成的关键酶。本研究测定了干旱胁迫下拟南芥叶片阿坝含量的变化，并对22份拟南芥材料的AtNCED 3基因进行了序列测定和比较。结果表明，干旱胁迫下阿坝含量增加倍数在不同品种间差异较大。在AtNCED 3区域发现了高密度的单核苷酸多态性（SNP）和插入/缺失（indel），平均每87.4bp有一个SNP，每502 bp有一个indel。编码区的核苷酸多样性显著低于非编码区。方差分析结果表明，第274位（PS）和第327位（PR）的氨基酸变异可能是导致163 av在干旱胁迫下阿坝含量增加的原因。
Drought tolerance is a comprehensive quantitative trait that is being understood further at the molecular genetic level. Abscisic acid (ABA) is the main drought-induced hormone that regulates the expression of many genes related to drought responses. 9-cis-epoxycarotenoid dioxygenase (NCED3) is thought to be a key enzyme in ABA biosynthesis. In this paper, we measured the ABA content increase under drought stress, and sequenced and compared the sequence of AtNCED3 among 22 Arabidopsis thaliana accessions. The results showed that the fold of ABA content increase under drought stress was highly variable among these accessions. High density single nucleotide polymorphism (SNP) and insertion/deletion (indel) were found in the AtNCED3 region, on average one SNP per 87.4 bp and one indel per 502 bp. Nucleotide diversity was significantly lower in the coding region than that in non-coding regions. The results of an association study with anova analysis suggested that the 274th site (P S) and the 327th site (P R) amino acid variations might be the cause of ABA content increase of 163av accession under drought stress.