Aligned fibrillar collagen matrices obtained by shear flow deposition
Aligned fibrillar collagen matrices obtained by shear flow deposition
复制标题
DOI：
10.1016/j.biomaterials.2008.06.016
复制
发表时间：
2008-10-01
期刊：
影响因子：
14
通讯作者：
Werner, Carsten
中科院分区：
文献类型：
--
作者：
Lanfer, Babette;Freudenberg, Uwe;Werner, Carsten
在这里，我们提出了一种新技术来生成具有不同结构特征的表面结合的 I 型胶原纤维基质。排列整齐的胶原纤维沉积在来自流经微流体通道系统的胶原溶液的平面基底上。胶原溶液浓度、凝胶化程度、剪切速率和基质的预涂被证明可以确定固定原纤维的方向和密度。使用共焦反射显微镜和原子力显微镜对获得的基质进行成像。应用图像分析技术来评估胶原原纤维的方向和覆盖范围。正如预期的那样，胶原原纤维取向的程度随着溶液流速的增加而增加，而基质密度在较高的胶原溶液浓度和疏水性聚合物预涂层上增加。此外，固定胶原原纤维的长度随着溶液浓度和胶凝时间的增加而增加。 (c) 2008 Elsevier Ltd. 保留所有权利。
Here we present a new technique to generate surface-bound collagen I fibril matrices with differing structural characteristics. Aligned collagen fibrils were deposited on planar Substrates from collagen solutions streaming through a microfluidic channel system. Collagen solution concentration, degree of gelation, shear rate and pre-coating of the Substrate were demonstrated to determine the orientation and density of the immobilized fibrils. The obtained matrices were imaged using confocal reflection microscopy and atomic force microscopy. Image analysis techniques were applied to evaluate collagen fibril orientation and coverage. As expected, the degree of collagen fibril orientation increased with increasing flow rates of the solution while the matrix density increased at higher collagen solution concentrations and on hydrophobic polymer pre-coatings. Additionally, length of the immobilized collagen fibrils increased with increasing solution concentration and gelation time. (c) 2008 Elsevier Ltd. All rights reserved.