Different isoforms of fasciclin II play distinct roles in the guidance of neuronal migration during insect embryogenesis.
Different isoforms of fasciclin II play distinct roles in the guidance of neuronal migration during insect embryogenesis.
复制标题
成束蛋白 II 的不同亚型在昆虫胚胎发生过程中指导神经元迁移中发挥不同的作用。
DOI：
10.1006/dbio.2000.9777
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Copenhaver,PF
中科院分区：
文献类型：
--
作者：
Wright,JW;Copenhaver,PF
在Manduca sexta的肠道神经系统（ENS）形成过程中，已确定的神经元和神经胶质细胞群体参与了精确定时的迁移波。细胞粘附受体fasciclin II在发育中的ENS中表达，是正常迁移所必需的。之前，我们鉴定了Manduca fasciclin II （MFas II）的两个亚型，一个是糖基磷脂酰肌醇连接亚型（GPI-MFas II），另一个是跨膜亚型（TM-MFas II）。利用RNA和抗体探针，我们发现这两种异构体以细胞类型特异性的模式表达：GPI-MFas II在胶质细胞和新生神经元中表达，而TM-MFas II仅限于分化神经元。每种异构体的表达也与不同细胞类型的运动状态相对应：GPI-MFas II在贴壁紧密或缓慢扩散的细胞上检测到，而TM-MFas II在积极迁移的神经元上表达，并定位于其最运动的区域。在胚胎培养中对每种异构体的操作表明，它们发挥着不同的作用：GPI-MFas II严格地作为粘附分子，而TM-MFas II促进EP细胞的运动并维持其通路的束控。这些结果表明，在发育中的神经系统中，精确调控的同种异构体表达模式控制着束状蛋白II的功能。
During the formation of the enteric nervous system (ENS) of the moth Manduca sexta, identified populations of neurons and glial cells participate in precisely timed waves of migration. The cell adhesion receptor fasciclin II is expressed in the developing ENS and is required for normal migration. Previously, we identified two isoforms of Manduca fasciclin II (MFas II), a glycosyl phosphatidylinositol-linked isoform (GPI-MFas II) and a transmembrane isoform (TM-MFas II). Using RNA and antibody probes, we found that these two isoforms were expressed in cell type-specific patterns: GPI-MFas II was expressed by glial cells and newly generated neurons, while TM-MFas II was confined to differentiating neurons. The expression of each isoform also corresponded to the motile state of the different cell types: GPI-MFas II was detected on tightly adherent or slowly spreading cells, while TM-MFas II was expressed by actively migrating neurons and was localized to their most motile regions. Manipulations of each isoform in embryo culture showed that they played distinct roles: whereas GPI-MFas II acted strictly as an adhesion molecule, TM-MFas II promoted the motility of the EP cells as well as maintaining fasciculation with their pathways. These results indicate that precisely regulated patterns of isoform expression govern the functions of fasciclin II within the developing nervous system.