Increase of bone-morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats.
Increase of bone-morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats.
复制标题
肺切除大鼠中表达骨形态发生蛋白 7 的肺常驻细胞增加。
DOI：
10.1007/s00595-013-0604-7
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发表时间：
2014
期刊：
影响因子：
2.5
通讯作者：
Maehara Y
中科院分区：
文献类型：
--
作者：
Ohba T;Wada H;Yoshino I;Yoshida S;Tagawa T;Shoji F;Yamazaki K;Maehara Y
目的：肺切除术后的啮齿类动物可发生代偿性肺生长（CLG），但CLG过程中再生组织的细胞来源仍不清楚。我们研究了肺驻留细胞的分化和参与的骨髓（BM）衍生细胞在残肺的pneumonectomized rats.MethodsAfter左肺切除术，右残肺的Wistar大鼠进行了形态学和分子实验在几个时间点。我们研究了骨形态发生蛋白7（BMP-7）的表达，上皮分化的加速剂，基于残肺的基因表达谱数据。接下来，我们评估了GFP阳性细胞在残余肺的Wistar大鼠，已接受骨髓移植绿色荧光蛋白（GFP）基因转基因Wistar大鼠之前，左pneumonectomy.ResultsWe观察到肺气肿的变化，基因表达谱的调制，和增殖细胞核抗原阳性细胞在肺泡的残余肺的进展。BMP-7蛋白阳性细胞在肺泡隔中检测到，随着时间的推移，肺气肿的进展，这显着增加。荧光显微镜，免疫组化，或聚合酶链反应在任何时间，在GFP-BM转移大鼠的右残肺中没有检测到骨髓来源的细胞。
PurposeCompensatory lung growth (CLG) is recognized in rodents subjected to major pulmonary resection; however, the source of cells constituting regenerated tissues during the CLG is still unknown. We investigated the differentiation of lung resident cells and the participation of bone marrow (BM)-derived cells in the remnant lung of pneumonectomized rats.MethodsAfter left pneumonectomy, the right remnant lung of Wistar rats was subjected to morphologic and molecular experiments at several time points. We studied the expression of bone morphogenic protein 7 (BMP-7), an accelerator of epithelial differentiation, based on the gene expression profile data of the remnant lung. Next, we evaluated the presence of GFP-positive cells in the remnant lung of Wistar rats that had received BM transplantation from green fluorescent protein (GFP) gene-transgenic Wistar rats prior to left pneumonectomy.ResultsWe observed progression of emphysematous change, modulation of gene expression profile, and proliferating cellular nuclear antigen-positive cells in the alveoli of the remnant lungs. BMP-7 protein positive cells were detected in the alveolar septa, which increased significantly over time with the progression of emphysematous change. No bone marrow-derived cells were detected in the right remnant lung of the GFP-BM transferred rats by fluorescence microscopy, immunohistochemistry, or polymerase chain reaction at any time.ConclusionLung resident cells appear to contribute to CLG, possibly via a trans-differentiation pathway.