NF-κB activation in peripheral blood mononuclear cells in neonatal asphyxia
NF-κB activation in peripheral blood mononuclear cells in neonatal asphyxia
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DOI：
10.1046/j.1365-2249.2003.02127.x
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发表时间：
2003-05-01
影响因子：
4.6
通讯作者：
Furukawa, S
中科院分区：
文献类型：
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作者：
Hasegawa, K;Ichiyama, T;Furukawa, S
新生儿窒息导致缺氧缺血性脑病。先前的研究已经证明，脑缺氧和缺血导致促炎细胞因子的产生，包括肿瘤坏死因子-α（TNF-α）、白细胞介素-1（IL-1）和IL-6。转录因子NF-κ B对于这些细胞因子的表达是必需的。我们通过流式细胞术检测了新生儿窒息时外周血单个核细胞（PBMC）中NF-κ B B是否活化。此外，我们还研究了PBMC中NF-κ B活化与神经预后的关系。流式细胞术分析显示，有神经系统后遗症的窒息患者的CD 14（+）单核/巨噬细胞中NF-κ B B活化水平显著高于对照组和存活的窒息患者（分别为31.7 +/-7.2%vs.2.5 +/-0.9%，P = 0.008和vs.1.6 +/-1.4%，P = 0.014）。我们的研究结果表明，NF-κ B B活化的外周血CD 14（+）单核细胞/巨噬细胞在新生儿窒息是重要的预测随后的神经系统后遗症。
Neonatal asphyxia results in hypoxic-ischaemic encephalopathy. Previous studies have demonstrated that brain hypoxia and ischaemia lead to the production of proinflammatory cytokines, including tumour necrosis factor-alpha (TNF-alpha ), interleukin-1 (IL-1) and IL-6. Transcription factor NF-kappa B is essential for the expression of these cytokines. We examined whether or not NF-kappa B is activated in peripheral mononuclear cells (PBMC) in neonatal asphyxia by flow cytometry. In addition, we examined the relationship between NF-kappa B activation in PBMC and the neurological prognosis. Flow cytometry analysis demonstrated that the level of NF-kappa B activation in CD14(+) monocytes/macrophages of the patients with asphyxia who had neurological sequelae was significantly higher than in the controls, and in the patients with asphyxia who survived (31.7 +/- 7.2%versus 2.5 +/- 0.9%, P = 0.008, and versus 1.6 +/- 1.4%, P = 0.014, respectively). Our findings suggest that NF-kappa B activation in peripheral blood CD14(+) monocytes/macrophages in neonatal asphyxia is important for predicting the subsequent neurological sequelae.