Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion
Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion
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DOI：
10.2220/biomedres.31.27
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发表时间：
2010-02-01
影响因子：
1.2
通讯作者：
Fukuoka, Hirotaro
中科院分区：
文献类型：
--
作者：
Park, Byung-Soon;Kim, Won-Serk;Fukuoka, Hirotaro
脂肪源性干细胞（ADSCs）及其分泌体介导多种皮肤再生作用，如伤口愈合和抗氧化保护，这些作用会因缺氧而增强。我们研究了脂肪干细胞条件培养基（CM）的毛发生长促进作用，以确定脂肪干细胞及其分泌物是否能再生毛发，以及缺氧是否能促进毛发再生。如果是这样的话，我们想确定负责缺氧增强毛发再生的因素。我们发现，ADSC-CM皮下给药诱导毛发生长期，并增加毛发再生C3 H/NeH小鼠。此外，ADSC-CM增加人毛囊毛乳头细胞（HFDPC）和人上皮角质形成细胞（HEK）的增殖，所述细胞来源于毛囊中存在的两种主要细胞类型。我们研究了缺氧油ADSC功能的影响，使用相同的动物模型，其中缺氧增加毛发再生。分析了在低氧或常氧条件下培养的ADSC-CM中的41种生长因子。缺氧使胰岛素样生长因子结合蛋白（IGFBP）-1、IGFBP-2、巨噬细胞集落刺激因子（M-CSF）、M-CSF受体、血小板衍生生长因子受体β和血管内皮生长因子的分泌明显增加，而上皮生长因子的分泌减少。这是合理的结论，脂肪干细胞促进毛发生长通过旁分泌机制，增强缺氧。
Adipose-derived stem cells (ADSCs) and their secretomes mediate diverse skin-regeneration effects, Such as wound-healing and antioxidant protection, that are enhanced by hypoxia. We investigated the hair-growth-promoting effect of conditioned medium (CM) of ADSCs to determine if ADSCs and their secretomes regenerate hair and if hypoxia enhances hair regeneration. If so, we wanted to identify the factors responsible for hypoxia-enhanced hair-regeneration. We found that ADSC-CM administrated subcutaneously induced the anagen phase and increased hair regeneration in C3H/NeH mice. In addition, ADSC-CM increased the proliferation Of human follicle dermal papilla cells (HFDPCs) and human epithelial keratinocytes (HEKs), which are derived from two major cell types present in hair follicles. We investigated the effect of hypoxia oil ADSC function using the same animal model in which hypoxia increased hair regrowth. Forty-one growth factors in ADSC-CM from Cells Cultured under hypoxic or normoxic conditions were analyzed. The secretion of insulin-like growth factor binding protein (IGFBP)-1, IGFBP-2, macrophage colony-stimulating factor (M-CSF), M-CSF receptor, platelet-derived growth factor receptor-beta, and vascular endothelial growth factor was significantly increased by hypoxia, while the secretion of epithelial growth factor production was decreased. It is reasonable to conclude that ADSCs promote hair growth via a paracrine mechanism that is enhanced by hypoxia.