Widespread occurrence of calcium-activated, phospholipid-dependent protein kinase in mammalian tissues.
Widespread occurrence of calcium-activated, phospholipid-dependent protein kinase in mammalian tissues.
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钙激活的磷脂依赖性蛋白激酶在哺乳动物组织中广泛存在。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
Yasutomi Nishizuka
中科院分区：
文献类型：
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作者：
R. Minakuchi;Y. Takai;Binzu Yu;Yasutomi Nishizuka
Ca2+激活的磷脂依赖性多功能蛋白激酶最初发现于大鼠脑中，在多种哺乳动物组织中存在。在大多数组织中，当用小牛胸腺H1组蛋白作为磷酸盐受体测定时，酶活性与环AMP依赖性蛋白激酶的活性相当。在某些组织如血小板、脑和淋巴细胞中，这种酶远远超过环腺苷酸依赖酶。在各种组织中发现的这种蛋白激酶显示出非常相似的物理、动力学和催化性质，并且似乎没有显示出组织和物种特异性。可以想象，这种蛋白激酶通过大量细胞外信使在跨膜控制蛋白磷酸化中发挥作用，这些信使诱导其靶组织中的磷脂酰肌醇周转。
Ca2+-activated, phospholipid-dependent multifunctional protein kinase originally found in rat brain occurs in a variety of mammalian tissues. In most tissues the enzyme activity is comparable to that of cyclic AMP-dependent protein kinase when assayed with calf thymus H1 histone as phosphate acceptor. In some tissues such as platelets, brain, and lymphocytes the enzyme far exceeds the cyclic AMP-dependent enzyme. This species of protein kinase found in various tissues shows very similar physical, kinetic, and catalytic properties, and does not appear to show tissue and species specificities. It is conceivable that this protein kinase plays roles in transmembrane control of protein phosphorylation by a large number of extracellular messengers which induce phosphatidylinositol turnover in their target tissues.