EXPRESSION OF HUMAN BONE-RELATED PROTEINS IN THE HEMATOPOIETIC MICROENVIRONMENT
EXPRESSION OF HUMAN BONE-RELATED PROTEINS IN THE HEMATOPOIETIC MICROENVIRONMENT
复制标题
DOI：
10.1172/jci114852
复制
发表时间：
1990-11-01
影响因子：
15.9
通讯作者：
MANN, KG
中科院分区：
文献类型：
--
作者：
LONG, MW;WILLIAMS, JL;MANN, KG
鉴于骨和骨髓之间的密切关系，我们假设人的骨髓可能是骨形成祖细胞的来源(或储存库)。我们观察到骨髓中的一群细胞，它们产生骨骼特异的或与骨骼相关的蛋白质。这些蛋白质的产生在人的长期骨髓细胞培养中受到发育调节；骨蛋白产生细胞(BPPC)是在无血清、短期培养条件下观察到的，对骨相关而不是造血生长因子有反应，并且来源于低密度、非贴壁、my10阴性(或低my10密度)的骨髓细胞(my10是大多数造血祖细胞上发现的抗原)。骨髓来源的BPPC在含血清的二次培养中培养，可使其分化为成骨样细胞。在发育的这个阶段，BPPC产生一种细胞外基质，其中包括骨相关蛋白和放射性标记的钙。因此，人骨髓BPPC代表了一种新描述的细胞表型，对骨和造血细胞生物学都很重要。
Given the intimate relationship between bone and bone marrow, we hypothesized that the human bone marrow may function as a source (or reservoir) of bone-forming progenitor cells. We observed a population of cells within the bone marrow which produce bone-specific or bone-related proteins. The production of these proteins was developmentally regulated in human long-term bone marrow cell cultures; the bone protein-producing cells (BPPC) are observed under serum-free, short-term culture conditions, respond to bone-related and not hematopoietic growth factors, and are derived from a population of low-density, nonadherent, My10-negative (or low My10 density), marrow cells (My10 is an antigen found on most hematopoietic progenitor cells). Cultivation of marrow-derived BPPC in secondary, serum-containing cultures results in their differentiation into osteoblastlike cells. At this stage of development, BPPC produce an extracellular matrix which incorporates both bone-related proteins and radiolabeled calcium. Human bone marrow BPPC thus represent a newly described cell phenotype important to both bone and hematopoietic cell biology.