Biosynthesis of coelenterazine in the deep-sea copepod, Metridia pacifica
Biosynthesis of coelenterazine in the deep-sea copepod, Metridia pacifica
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DOI：
10.1016/j.bbrc.2009.10.028
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发表时间：
2009-12-18
影响因子：
3.1
通讯作者：
Inouye, Satoshi
中科院分区：
文献类型：
--
作者：
Oba, Yuichi;Kato, Shin-ichi;Inouye, Satoshi
腔肠素是一种咪唑并吡嗪酮类化合物（3，7-二氢咪唑并吡嗪-3-酮结构），广泛分布于海洋生物中，用作多种生物发光反应的荧光素。我们已经使用电喷雾电离离子阱质谱研究是否深海发光桡足类Metridia pacifica能够合成腔肠素。用氘标记的L-酪氨酸和L-苯丙氨酸进行的摄食实验表明，腔肠素可以由两分子L-酪氨酸和一分子L-苯丙氨酸在M. pacifica。这是首次证明腔肠素是由海洋生物中的游离L-氨基酸生物合成的。(C)2009 Elsevier Inc. All rights reserved.
Coelenterazine is an imidazopyrazinone compound (3,7-dihydroimidazopyrazin-3-one structure) that is widely distributed in marine organisms and used as a luciferin for various bioluminescence reactions. We have used electrospray ionization-ion trap-mass spectrometry to investigate whether the deep-sea luminous copepod Metridia pacifica is able to synthesize coelenterazine. By feeding experiments using deuterium labeled amino acids of L-tyrosine and L-phenylalanine, we have shown that coelenterazine can be synthesized from two molecules of L-tyrosine and one molecule of L-phenylalanine in M. pacifica. This is the first demonstration that coelenterazine is biosynthesized from free L-amino acids in a marine organism. (C) 2009 Elsevier Inc. All rights reserved.