Effects of mixing intensity on cell seeding and proliferation in three-dimensional fibrous matrices
Effects of mixing intensity on cell seeding and proliferation in three-dimensional fibrous matrices
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DOI：
10.1002/bit.21091
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发表时间：
2007-02-01
影响因子：
3.8
通讯作者：
Yang, Shang-Tian
中科院分区：
文献类型：
--
作者：
Ouyang, Anli;Yang, Shang-Tian
非织造纤维基质已广泛用于细胞和组织培养，因为其具有大表面积和孔隙空间的三维（3-D）结构可以支持高密度细胞生长。虽然细胞粘附和生长的2-D表面上已被彻底调查，很少有人知道细胞培养在3-D矩阵。因此，研究了混合强度对纤维基质中细胞接种、粘附和生长的影响。采用动态接种法和静态接种法将人卵巢癌细胞和骨肉瘤细胞接种于聚对苯二甲酸乙二酯非织造布基质中，动态接种法的接种效率和细胞在基质中的分布均上级静态接种法。动态排种使排种效率从40%左右提高到90%以上。当施加更高的混合强度时，细胞附着和脱离速率都增加。细胞的附着是运输限制的，如所示的增加的附着率与细胞的传质系数的增加。同时，细胞从3-D基质中脱离可以通过Bell模型来描述。研究了基质孔径对细胞粘附和增殖的影响。一般来说，较小的孔径有利于细胞的附着和增殖。进一步的分析表明，混合强度和孔径之间的相互作用起着至关重要的作用，在流体动力学损伤细胞，这被发现是显着的Kolomogorov涡的尺寸小于基质孔隙。增加混合强度也增加了氧传递，降低了葡萄糖的乳酸产量，并改善了细胞生长。
Nonwoven fibrous matrices have been widely used in cell and tissue cultures because their three-dimensional (3-D) structures with large surface areas and pore spaces can support high-density cell growth. Although cell adherence and growth on 2-D surfaces have been thoroughly investigated, very little is known for cells cultured in 3-D matrices. The effects of mixing intensity on cell seeding, adherence, and growth in fibrous matrices were thus investigated. Chinese Hamster Ovary and osteosarcoma cells were inoculated into nonwoven polyethylene terephthalate matrices by dynamics and static seeding methods, of which the former was found to be superior in seeding efficiency and cell distribution in the matrices. Dynamic seeding increased seeding efficiency from similar to 40% to more than 90%. When higher mixing intensities were applied, both cell attachment and detachment rates increased. Cell attachment was transport limited, as indicated by the increased attachment rate with increasing the mass transfer coefficient of the cells. Meanwhile, cell detachment from the 3-D matrix can be described by the Bell model. The effects of matrix pore size on cell adherance and proliferation were also investigated. In general, the smaller pore size is favorable to cell attachment and proliferation. Further analysis revealed that the interaction between mixing intensity and pore size played a vital role in hydrodynamic damage to cells, which was found to be significant when the Kolomogorov eddy size was smaller than the matrix pores. Increasing mixing intensity also increased oxygen transfer, decreased the lactate yield from glucose, and improved cell growth.