Mass transfer trends occurring in engineered ex vivo tissue scaffolds
Mass transfer trends occurring in engineered ex vivo tissue scaffolds
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DOI：
10.1002/jbm.a.34092
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发表时间：
2012-08-01
影响因子：
4.9
通讯作者：
McFetridge, Peter
中科院分区：
文献类型：
--
作者：
Moore, Marc;Sarntinoranont, Malisa;McFetridge, Peter
在体内，脉管系统为细胞营养素提供了有效的递送系统;然而，人工支架没有这种机制，并且随后的传质限制导致有限的再生。在这些研究中，通过来自人脐静脉（HUV）的模型支架发生的区域传质特性进行了评估。我们的目的是定义与这些区域变化相关的异质性行为，并确定不同的脱细胞技术是否可以调节运输条件，以改善微环境条件，增强细胞整合。评估了三种脱细胞方法[Triton X-100（TX 100）、十二烷基硫酸钠（SDS）和丙酮/乙醇（ACE/EtOH）]对传质、细胞迁移、增殖和代谢活性的影响。结果表明，组织结构和组成的区域变化显着影响传质和细胞功能。与TX 100或SDS脱细胞切片相比，ACE/EtOH脱细胞处理增加了通过内膜和近中端区域（0250 μ m）的白蛋白质量通量;尽管使用TX 100时，整个组织厚度的所有区域的质量通量保持不变。相对于SDS脱细胞样品，用TX 100脱细胞的支架显示出进一步促进细胞迁移高达146%。这些结果表明，取决于支架衍生和细胞整合的预期，脱细胞化学的特异性影响支架分子结构，导致对传质和细胞反应的可变影响。(C)2012 Wiley Periodicals，Inc. J Biomed Mater Res Part A，2012。
In vivo the vasculature provides an effective delivery system for cellular nutrients; however, artificial scaffolds have no such mechanism, and the ensuing limitations in mass transfer result in limited regeneration. In these investigations, the regional mass transfer properties that occur through a model scaffold derived from the human umbilical vein (HUV) were assessed. Our aim was to define the heterogeneous behavior associated with these regional variations, and to establish if different decellularization technologies can modulate transport conditions to improve microenvironmental conditions that enhance cell integration. The effect of three decellularization methods [Triton X-100 (TX100), sodium dodecyl sulfate (SDS), and acetone/ethanol (ACE/EtOH)] on mass transfer, cellular migration, proliferation, and metabolic activity were assessed. Results show that regional variation in tissue structure and composition significantly affects both mass transfer and cell function. ACE/EtOH decellularization was shown to increase albumin mass flux through the intima and proximate-medial region (0250 mu m) when compared with sections decellularized with TX100 or SDS; although, mass flux remained constant over all regions of the full tissue thickness when using TX100. Scaffolds decellularized with TX100 were shown to promote cell migration up to 146% further relative to SDS decellularized samples. These results show that depending on scaffold derivation and expectations for cellular integration, specificities of the decellularization chemistry affect the scaffold molecular architecture resulting in variable effects on mass transfer and cellular response. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.