Impact of feeding system and infection status of the blood meal on Ixodes ricinus feeding
Impact of feeding system and infection status of the blood meal on Ixodes ricinus feeding
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DOI：
10.1016/j.ttbdis.2013.12.008
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发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Bonnet, Sarah I.
中科院分区：
文献类型：
--
作者：
Liu, Xiang Ye;Cote, Martine;Bonnet, Sarah I.
人工膜饲养系统是蜱饲养和研究蜱传病原体传播的有效工具。为了比较不同饲养系统对蜱饱食的影响，用人工膜饲养系统和活小鼠饲养蓖麻硬蜱。将绵羊血和鸡血与膜系统一起使用，以评估血液来源对蜱充血的影响。为了研究血粉感染对蜱虫充血的影响，通过膜喂养和小鼠喂养蜱虫感染巴尔通体或未感染的血液。评估了充血蜱的比例、蜱喂食持续时间和充血蜱的重量。在人工系统上喂食导致比在小鼠上喂食时更长的蜱喂食持续时间和更低比例的饱食蜱，然而，饱食蜱的重量不受影响。饱食蜱的比例和重量以及进食持续时间不受血液来源的影响。喂食受感染的血粉或受感染的小鼠可降低饱食蜱虫的比例和重量，但不影响蜱虫喂食的持续时间。(C)2014 Elsevier GmbH. All rights reserved.
Artificial membrane feeding systems are effective tools for both tick rearing and studying tick-borne pathogen transmission. In order to compare the effects of the type of feeding system on tick engorgement, Ixodes ricinus ticks were either fed on an artificial membrane feeding system or on live mice. Sheep and chicken blood were used with the membrane system to assess the effects of blood origin on tick engorgement. To investigate the effects of blood meal infection on tick engorgement, ticks were either fed with Bartonella-infected or uninfected blood, both via membrane feeding and on mice. The proportion of engorged ticks, the duration of tick feeding, and the weight of engorged ticks were assessed. Feeding on the artificial system led to a longer duration of tick feeding and a lower proportion of engorged ticks than when fed on mice, however, the weight of engorged ticks was unaffected. The proportion and weight of engorged ticks, as well as the duration of feeding were not affected by blood origin. Feeding on an infected blood meal or on infected mice decreased the proportion and the weight of engorged ticks, but did not affect tick feeding duration. (C) 2014 Elsevier GmbH. All rights reserved.