Dynamic and Combinatorial Landscape of Histone Modifications during the Intraerythrocytic Developmental Cycle of the Malaria Parasite.
Dynamic and Combinatorial Landscape of Histone Modifications during the Intraerythrocytic Developmental Cycle of the Malaria Parasite.
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DOI：
10.1021/acs.jproteome.6b00366
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发表时间：
2016-08-05
影响因子：
4.4
通讯作者：
Le Roch KG
中科院分区：
文献类型：
--
作者：
Saraf A;Cervantes S;Bunnik EM;Ponts N;Sardiu ME;Chung DW;Prudhomme J;Varberg JM;Wen Z;Washburn MP;Florens L;Le Roch KG
A major obstacle in understanding the complex biology of the malaria parasite remains to discover how gene transcription is controlled during its life cycle. Accumulating evidence indicates that the parasite’s epigenetic state plays a fundamental role in gene expression and virulence. Using a comprehensive and quantitative mass spectrometry approach, we determined the global and dynamic abundance of histones and their covalent post-transcriptional modifications throughout the intra-erythrocytic developmental cycle of Plasmodium falciparum. We detected a total of 232 distinct modifications, of which 160 had never been detected in Plasmodium and 88 had never been identified in any other species. We further validated over 10% of the detected modifications and their expression patterns by multiple reaction monitoring assays. In addition, we uncovered an unusual chromatin organization with parasite-specific histone modifications and combinatorial dynamics that may be directly related to transcriptional activity, DNA replication, and cell cycle progression. Overall, our data suggest that the malaria parasite has a unique histone modification signature that correlates with parasite virulence.
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影响因子：
6.7
作者：
Ganesan K;Ponmee N;Jiang L;Fowble JW;White J;Kamchonwongpaisan S;Yuthavong Y;Wilairat P;Rathod PK
通讯作者：
Rathod PK
影响因子：
3.6
作者：
Hoeijmakers WA;Salcedo-Amaya AM;Smits AH;Françoijs KJ;Treeck M;Gilberger TW;Stunnenberg HG;Bártfai R
通讯作者：
Bártfai R
影响因子：
7
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Ay F;Bunnik EM;Varoquaux N;Bol SM;Prudhomme J;Vert JP;Noble WS;Le Roch KG
通讯作者：
Le Roch KG
影响因子：
4.8
作者：
Garcia, Benjamin A.;Hake, Sandra B.;Hunt, Donald F.
通讯作者：
Hunt, Donald F.
影响因子：
3.7
作者：
Britton, Laura-Mae P.;Newhart, Alyshia;Garcia, Benjamin A.
通讯作者：
Garcia, Benjamin A.