Tumor necrosis factor in the human fetoplacentary unit.
Tumor necrosis factor in the human fetoplacentary unit.
复制标题
人类胎儿胎盘单位的肿瘤坏死因子。
DOI：
10.1387/ijdb.2485698
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
M. Jäättelä
中科院分区：
文献类型：
--
作者：
E. Saksela;M. Jäättelä
在本文中，我们回顾了我们对人类胎盘和肾上腺组织中肿瘤坏死因子(TNF)的研究结果，并提出了这种多功能淋巴因子可能的新的生物学作用。妊娠中期和晚期羊水中的肿瘤坏死因子平均浓度为1.7~2.5 ng/ml。在羊水中，肿瘤坏死因子以生物非活性形式存在，而在新鲜制备的胎盘和蜕膜组织匀浆上清液中，根据肿瘤坏死因子对WEHI细胞的细胞毒作用，也发现了相应的水平。在其他胎儿组织的筛选中，我们也可以在胎儿肾上腺匀浆中检测到生物活性的肿瘤坏死因子，培养的胎儿肾上腺细胞在ACTH刺激下产生肿瘤坏死因子。通过抑制ACTH刺激培养的胎儿肾上腺皮质醇的合成，并将类固醇的合成转向脱氢表雄酮(DHEA)及其硫酸盐(DHEAS)，提示了其可能的生物学作用。这些都是胎盘类固醇合成的重要前体，而胎盘类固醇合成主要由正常妊娠期间的胎儿肾上腺提供，这一结果表明，肿瘤坏死因子在调节人胎儿-胎盘单位的类固醇合成中发挥了作用。
In the present paper we review our findings on tumor necrosis factor (TNF) in human placental and adrenal tissues and suggest a possible novel biological role for this multi-functional lymphokine. TNF was found in the second and third trimester amniotic fluids in concentrations varying from 1.7 to 2.5 ng/ml on the average. In the amniotic fluid TNF was present in a biologically inactive form but in freshly-prepared supernatants of placental and decidual tissue homogenates corresponding levels were also found with bioassays based on the cytotoxicity of TNF to WEHI cells. In screening other fetal tissues we could detect biologically active TNF also in homogenates of fetal adrenals, and cultured fetal adrenal cells produced TNF upon stimulation with ACTH. A possible biological function for TNF was suggested by the findings that is suppressed the ACTH stimulated cortisol synthesis of fetal adrenal cultures and switched the steroidogenesis towards dehydroepiandrosterone (DHEA) and its sulphate (DHEAS). These are important precursors for placental steroid synthesis which are mainly provided by the fetal adrenals during normal gestation, and the results thus suggest a role for TNF in the regulation of steroidogenesis in the human fetoplacentary unit.