HUMAN HERPESVIRUS-7 IS A T-LYMPHOTROPIC VIRUS AND IS RELATED TO, BUT SIGNIFICANTLY DIFFERENT FROM, HUMAN HERPESVIRUS-6 AND HUMAN CYTOMEGALOVIRUS
HUMAN HERPESVIRUS-7 IS A T-LYMPHOTROPIC VIRUS AND IS RELATED TO, BUT SIGNIFICANTLY DIFFERENT FROM, HUMAN HERPESVIRUS-6 AND HUMAN CYTOMEGALOVIRUS
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DOI：
10.1073/pnas.89.21.10552
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
GALLO, RC
中科院分区：
文献类型：
--
作者：
BERNEMAN, ZN;ABLASHI, DV;GALLO, RC
从一名慢性疲劳综合症 (CFS) 患者身上分离出人类疱疹病毒 7 型 (HHV-7) 的独立毒株 (JI)。血清流行病学无法确定 HHV-7 和 CFS 之间存在显着关联。 HHV-7 是一种嗜 T 淋巴细胞病毒，感染 CD4+ 和 CD8+ 原代淋巴细胞。 HHV-7 还可以感染 SUP-T1（一种未成熟的 T 细胞系），但成功率参差不齐。使用 DNA 探针扫描 58.8% 的人类疱疹病毒 6 (HHV-6) 基因组并与迄今为止测试的所有 HHV-6 毒株杂交进行 Southern 印迹分析，显示与 HHV-7 的同源性仅占探针总长度的 37.4%。 HHV-7 含有 GGGTTA 重复序列，HHV-6 和马立克氏病鸡疱疹病毒也是如此。 HHV-7(JI) 186 个碱基对片段的 DNA 测序显示，其与 HHV-6 和人巨细胞病毒的同一性分别为 57.5% 和 36%。源自该序列的寡核苷酸引物（HV7/HV8、HV10/HV11）仅扩增 HHV-7 DNA，不扩增其他人类疱疹病毒（包括 12 种不同的 HHV-6 毒株）的 DNA。使用含有仅与 HHV-7 DNA 杂交的 186 碱基对 HHV-7 DNA 片段的 p43L3 探针进行 Southern 印迹分析。一方面，人巨细胞病毒与 HHV-6 和 HHV-7 之间的分子差异大于 HHV-6 和 HHV-7 之间的差异，而后者又大于 HHV-6 毒株之间的差异。这项研究支持将 HHV-7 分类为人类 β-疱疹病毒的另一成员。
An independent strain (JI) of human herpesvirus 7 (HHV-7) was isolated from a patient with chronic fatigue syndrome (CFS). No significant association could be established by seroepidemiology between HHV-7 and CFS. HHV-7 is a T-lymphotropic virus, infecting CD4+ and CD8+ primary lymphocytes. HHV-7 can also infect SUP-T1, an immature T-cell line, with variable success. Southern blot analysis with DNA probes scanning 58.8% of the human herpesvirus 6 (HHV-6) genome and hybridizing to all HHV-6 strains tested so far revealed homology to HHV-7 with only 37.4% of the total probe length. HHV-7 contains the GGGTTA repetitive sequence, as do HHV-6 and Marek's disease chicken herpesvirus. DNA sequencing of a 186-base-pair fragment of HHV-7(JI) revealed an identity with HHV-6 and human cytomegalovirus of 57.5% and 36%, respectively. Oligonucleotide primers derived from this sequence (HV7/HV8, HV10/HV11) amplified HHV-7 DNA only and did not amplify DNA from other human herpesviruses, including 12 different HHV-6 strains. Southern blot analysis with the p43L3 probe containing the 186-base-pair HHV-7 DNA fragment hybridized to HHV-7 DNA only. The molecular divergence between human cytomegalovirus, on the one hand, and HHV-6 and HHV-7, on the other, is greater than between HHV-6 and HHV-7, which, in turn, is greater than the difference between HHV-6 strains. This study supports the classification of HHV-7 as an additional member of the human beta-herpesviruses.