Cloning and pattern of gsdf mRNA during gonadal development in the protogynous Epinephelus akaara
Cloning and pattern of gsdf mRNA during gonadal development in the protogynous Epinephelus akaara
复制标题
赤带石斑鱼性腺发育过程中 gsdf mRNA 的克隆和模式。
DOI：
10.1016/j.anireprosci.2015.12.004
复制
发表时间：
2016-02-01
影响因子：
2.2
通讯作者：
Chen, Shi Xi
中科院分区：
文献类型：
--
作者：
Chen, Yuan;Hong, Wan Shu;Chen, Shi Xi
性腺体细胞衍生因子（gsdf）是一种硬骨鱼和性腺特异性生长因子，参与早期生殖细胞的发育。赤点石斑鱼是一种雌雄同体的鱼类，为研究硬骨鱼类的性别决定和分化机制提供了一种新的模型。本研究从大肠杆菌中克隆了一个长2307 bp的gsdf基因。并进一步分析了其在雌性期和性转变发育期的组织分布和性腺基因表达模式。并分析了gsdf在过渡发育后期的细胞定位。同时测定血清性类固醇激素（E-2、11-Kr和DHP）浓度。gsdf转录本只定位于性腺。在雌性阶段的早期发育阶段，当卵精巢主要包含卵原细胞和初级生长卵母细胞，gsdf mRNA相对更丰富。然而，随着卵母细胞发育的进行，gsdf的相对丰度逐渐降低，并且持续降低。在过渡期，gsdf mRNA的相对丰度在发育早期略有增加，在发育后期进一步增加，并在发育早期精原细胞周围的支持细胞中观察到gsdf转录本。在性类固醇激素中，11-KT浓度与性别转变过程中gsdf mRNA的量呈正相关。这些结果表明，gsdf可能在调节减数分裂前生殖细胞增殖中发挥作用，并参与大肠杆菌的性别转变。阿卡拉(C)2015 Elsevier B. V.版权所有。
Gonadal soma-derived factor (gsdf) is a teleost- and gonad-specific growth factor involved in early germ cell development. The red spotted grouper, Epinephelus akaara, as a protogynous hermaphrodite, provides a novel model for understanding the mechanisms of sex determination and differentiation in teleosts. In the present study, a 2307-bp long gsdf gene was cloned from E. akaara and there was further analysis of its tissue distribution and gonadal patterns of gene expression during the female phase and sex change developmental stages. The cellular localization of gsdf at the late transitional developmental stage was also analyzed. In addition, the concentrations of serum sex steroid hormones (E-2, 11-Kr and DHP) were determined. The gsdf transcripts were exclusively localized in the gonad. During the female phase at an early developmental stage, when the ovotestis contained mainly oogonia and primary growth oocytes, the gsdf mRNA was relatively more abundant. The relative abundance of gsdf decreased, however, and the lesser amount was sustained with the advancement of oocyte development. During the transitional phase, the relative abundance of gsdf mRNA increased slightly at the early developmental stage and there were further increases in relative abundance in the late developmental stage, and the gsdf transcripts were observed in the Sertoli cells surrounding early developing spermatogonia. Among the sex steroids, 11-KT concentrations were positively correlated with amount of gsdf mRNA during sex change. These results suggest that gsdf could have roles in regulating pre-meiotic germ cell proliferation and be involved in sex change in E. akaara. (C) 2015 Elsevier B.V. All rights reserved.