Annotation and analysis of malic enzyme genes encoding for multiple isoforms in the fungus Mucor circinelloides CBS 277.49
Annotation and analysis of malic enzyme genes encoding for multiple isoforms in the fungus Mucor circinelloides CBS 277.49
复制标题
真菌卷枝毛霉 CBS 277.49 中编码多种亚型的苹果酸酶基因的注释和分析
DOI：
10.1007/s10529-012-0859-x
复制
发表时间：
2012-05-01
影响因子：
2.7
通讯作者：
Song, Yuanda
中科院分区：
文献类型：
--
作者：
Vongsangnak, Wanwipa;Zhang, Yingtong;Song, Yuanda
基于卷枝毛霉CBS 277.49的基因组数据，我们对5个苹果酸酶基因进行了注释：它们都编码含有苹果酸结合、NAD+结合和NAD（P）+结合保守结构域的蛋白质。苹果酸酶基因的系统发育分析表明，基因ID 78524和11639共享~80%的氨基酸同一性，并被分组在簇1中;基因ID 182779、186772和116127共享~66%的氨基酸同一性，被分组在簇2中。基因ID 78524、11639和166127产生定位于胞浆中的蛋白质，而来自基因182779和186772的产物定位于胞质溶胶中。基于Song等人先前发表的比较分析（Microbiology 147：1507-1515，2001），我们提出苹果酸酶基因ID 78524、166127、182779、186772、11639分别代表蛋白质同种型I、II、III/IV、V和VI。
Based on the newly-released genomic data of Mucor circinelloides CBS 277.49, we have annotated five genes encoding for malic enzyme: all code for proteins that contain conserved domains/motifs for malic acid binding, NAD+ binding and NAD(P)+ binding. Phylogenetic analysis for malic enzyme genes showed that genes ID 78524 and 11639 share ~80% amino acid identity and are grouped in cluster 1; genes ID 182779, 186772 and 116127 share ~66% amino acid identity are grouped in cluster 2. Genes ID 78524, 11639 and 166127 produce proteins that are localized in the mitochondrion, while the products from genes 182779 and 186772 are localized in the cytosol. Based on the comparative analysis published previously by Song et al. (Microbiology 147:1507–1515, 2001), we propose that malic enzyme genes ID 78524, 166127, 182779, 186772, 11639, respectively, represent protein isoforms I, II, III/IV, V, and VI.