Normal and reactive NG2+ glial cells are distinct from resting and activated microglia
Normal and reactive NG2+ glial cells are distinct from resting and activated microglia
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DOI：
10.1002/(sici)1097-4547(19970515)48:4
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发表时间：
1997-05
影响因子：
4.2
通讯作者：
A. Nishiyama;Min Yu;J. Drazba;V. Tuohy
中科院分区：
文献类型：
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作者：
A. Nishiyama;Min Yu;J. Drazba;V. Tuohy
我们之前曾使用 NG2 蛋白多糖和血小板衍生生长因子 α 受体（PDGF α 受体）的抗体在体内和体外鉴定少突胶质祖细胞。最近发现，广泛用作少突胶质细胞祖细胞标记物的 GD3 抗原也由小胶质细胞表达。在这项研究中，我们研究了正常发育和成熟大鼠脑中以及实验性自身免疫性脑脊髓炎 (EAE) 小鼠炎症病变中 NG2+/PDGF α 受体+ 胶质祖细胞和小胶质细胞之间的关系。使用抗 NG2 抗体和加纳单叶植物凝集素 (GSA I-B4) 或单克隆抗体 4H1 对正常大鼠大脑切片进行双重标记，表明 NG2+ 神经胶质祖细胞和中枢神经系统实质中的小胶质细胞之间不存在重叠。在 EAE 病变中，通过 F4/80 和 CD45 抗体识别的 NG2+ 细胞和小胶质细胞均表现出反应性变化，其特征是细胞数量和染色强度增加以及细胞突起缩短和增厚。这两种细胞类型均被发现围绕血管周围淋巴细胞浸润。 NG2 和 F4/80 或 CD45 的双标记 EAE 切片未能揭示共表达两种抗原的细胞，这表明反应性 NG2+ 细胞与激活的小胶质细胞不同。然而，在正常大脑中观察到 NG2+ 细胞和小胶质细胞之间存在密切的空间关系，并且在 EAE 中观察到更大程度的关系，其中有时会看到激活的小胶质细胞的过程包围着 NG2+ 细胞。这些观察结果表明小胶质细胞和 NG2+ 胶质细胞之间存在功能性相互作用。 J.神经科学。资源。 48:299–312, 1997。© 1997 Wiley-Liss, Inc.
We have previously used antibodies to the NG2 proteoglycan and the alpha receptor for platelet‐derived growth factor (PDGF α receptor) to identify oligodendroglial progenitor cells in vivo and in vitro. It has recently become evident that the GD3 antigen, which has been widely used as a marker for oligodendrocyte progenitor cells, is also expressed by microglial cells. In this study we have examined the relationship between the NG2+/PDGF α receptor+ glial progenitor cells and microglial cells in normal developing and mature rat brain and in inflammatory lesions in mice with experimental autoimmune encephalomyelitis (EAE). Double‐labeling of sections from normal rat brain using anti‐NG2 antibodies and lectin from Griffonia simplicifolia (GSA I‐B4) or monoclonal antibody 4H1 indicated that there is no overlap between NG2+ glial progenitor cells and microglia in the parenchyma of the central nervous system. In EAE lesions, both NG2+ cells and microglia, identified by antibodies to F4/80 and CD45, displayed reactive changes characterized by increased cell number and staining intensity and shortening and thickening of cell processes. Both cell types were found surrounding perivascular infiltrates of lymphocytes. Double‐labeling EAE sections for NG2 and F4/80 or CD45 failed to reveal cells that co‐expressed both antigens, suggesting that reactive NG2+ cells are distinct from activated microglia. However, a close spatial relationship between NG2+ cells and microglia was observed in the normal brain and to a greater extent in EAE, where processes of an activated microglial cell were sometimes seen to encircle an NG2+ cell. These observations are indicative of a functional interaction between microglia and the NG2+ glial cells. J. Neurosci. Res. 48:299–312, 1997. © 1997 Wiley‐Liss, Inc.