Distinct Roles of Met and Interacting Proteins on the Expressions of takeout Family Genes in Brown Planthopper.
Distinct Roles of Met and Interacting Proteins on the Expressions of takeout Family Genes in Brown Planthopper.
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蛋氨酸和相互作用蛋白对褐飞虱外带家族基因表达的不同作用
DOI：
10.3389/fphys.2017.00100
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发表时间：
2017
影响因子：
4
通讯作者：
Jiang Y
中科院分区：
文献类型：
--
作者：
Lin X;Zhang L;Jiang Y
takeout家族基因编码与嗅觉相关的相对较小的蛋白质，并受保幼激素（JH）调节。takeout基因调节各种生理过程，例如飞蝗的行为可塑性和果蝇的进食和求偶行为。因此，为了理解这些生理过程的调节机制，研究JH信号调节的takeout基因的表达是重要的。利用实时荧光定量PCR（qRTPCR）技术研究了JH信号在褐飞虱takeout家族基因调控中的作用。通过应用保幼激素III（JHIII）和下调JH信号传导途径中的关键基因来抑制褐飞虱）。局部施用JHIII诱导了大多数takeout家族基因的表达，并且在施用JHIII后2天和3天，其表达降低。通过RNAi下调褐飞虱JH受体NlMethoprene-tolerant（NlMet）及其相互作用伴侣NlTaiman（NlTai）和NlTaiman-Ftz-F1（NlTaiman-Ftz）对takeout家族基因的表达表现出明显的影响。NlMet和NlKrüppel-homolog 1（NlKr-h1）的下调增加了takeout家族基因的表达，而Met相互作用伴侣NlTai和NlKrüppel-Ftz的下调降低了大多数takeout家族基因的表达。这一工作进一步加深了我们对JH信号转导的分子功能和调控机制的理解。
The takeout family genes encode relatively small proteins that are related to olfaction and are regulated by juvenile hormone (JH). The takeout genes modulate various physiological processes, such as behavioral plasticity in the migratory locust Locusta migraloria and feeding and courtship behaviors in Drosophila. Therefore, to understand the regulatory mechanism of these physiological processes, it is important to study the expressions of the takeout genes that are regulated by JH signaling. We used quantitative real-time PCR (qRTPCR) to study the role of JH signaling in the regulation of the takeout family genes in the brown planthopper Nilaparvata lugens (N. lugens) through the application of Juvenile hormone III (JHIII) and the down-regulation of key genes in the JH signaling pathway. The topical application of JHIII induced the expressions of most of the takeout family genes, and their expressions decreased 2 and 3 days after the JHIII application. Down-regulating the brown planthopper JH receptor NlMethoprene-tolerant (NlMet) and its interacting partners, NlTaiman (NlTai) and Nlß-Ftz-F1 (Nlß-Ftz), through RNAi, exhibited distinct effects on the expressions of the takeout family genes. The down-regulation of NlMet and NlKrüppel-homolog 1 (NlKr-h1) increased the expressions of the takeout family genes, while the down-regulation of the Met interacting partners NlTai and Nlß-Ftz decreased the expressions of most of the takeout family genes. This work advanced our understanding of the molecular function and the regulatory mechanism of JH signaling.