The α6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad
The α6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad
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Frankliniella occidentalis 的 α6 烟碱乙酰胆碱受体亚基不参与多杀菌素耐药性
DOI：
10.1016/j.pestbp.2014.03.004
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发表时间：
2014-05-01
影响因子：
4.7
通讯作者：
Scott, Jeffrey G.
中科院分区：
文献类型：
--
作者：
Hou, Wenjie;Liu, Qiulei;Scott, Jeffrey G.
昆虫进化出抗药性，限制了杀虫剂的可持续使用。多杀菌素是一类环保型大环内酯类杀虫剂，也不例外。本研究调查了西花蓟马（Frankliniella occidentalis）（西花蓟马，WFT）对多杀菌素（最常见的多杀菌素杀虫剂）的遗传模式和抗性机制。耐药性（170,000倍）是常染色体且完全隐性遗传。最近的研究表明，烟碱乙酰胆碱受体α 6 亚基基因的缺失导致果蝇、小菜蛾和橘小实蝇菌株对多杀菌素产生抗药性，这表明nAChR α 6 亚基可能对该杀虫剂的毒性作用很重要。相反，最近提出 F. occidentalis 中该亚基的 G275E 突变是对多杀菌素的耐药机制。我们从来自中国和美国的三种易感菌株和两种多杀菌素耐药菌株中克隆并鉴定了 nAChR α 6。 Fo α 6 cDNA 为 1873 bp，开放阅读框为 1458 bp，编码 485 个氨基酸残基，预测分子量为 53.5 kDa，5' 和 3' UTR 分别为 121 和 294 bp。抗性蓟马和易感蓟马之间的 cDNA 序列没有差异，表明 G275E 突变并不赋予这些种群抗性。分离出由选择性剪接产生的十种 Foa6 亚型，并且在多杀菌素敏感菌株和耐药菌株之间没有差异。实时定量PCR分析显示Foα6在一龄幼虫、蛹和成虫中高表达，表达量分别是二龄幼虫的3.67、2.47、1.38倍。敏感菌株和抗性菌株之间的表达水平没有显着差异。这些结果表明Foa6不参与来自中国和美国的西花蓟马对多杀菌素的抗性。 (C) 2014 Elsevier Inc. 保留所有权利。
Insects evolve resistance which constrains the sustainable use of insecticides. Spinosyns, a class of environmentally-friendly macrolide insecticides, is not an exception. The mode of inheritance and the mechanisms of resistance to spinosad (the most common spinosyn insecticide) in Frankliniella occidentalis (Western flower thrips, WFT) were investigated in this study. Resistance (170,000-fold) was autosomal and completely recessive. Recent studies showed that deletion of the nicotinic acetylcholine receptor alpha 6 subunit gene resulted in strains of Drosophila melanogaster, Plutella xylostella and Bactrocera dorsalis that are resistant to spinosad, indicating that nAChR alpha 6 subunit maybe important for the toxic action of this insecticide. Conversely, a G275E mutation of this subunit in F. occidentalis was recently proposed as the mechanism of resistance to spinosad. We cloned and characterized nAChR alpha 6 from three susceptible and two spinosad resistant strains from China and the USA. The Fo alpha 6 cDNA is 1873 bp and the open reading frame is 1458 bp which encodes 485 amino acid residues with a predicted molecular weight of 53.5-kDa, the 5' and 3' UTRs are 121 and 294 bp, respectively. There was no difference in the cDNA sequence between the resistant and susceptible thrips, suggesting the G275E mutation does not confer resistance in these populations. Ten isoforms of Foa6, arising from alternative splicing, were isolated and did not differ between the spinosad-susceptible and resistant strains. Quantitative real time PCR analysis showed Fo alpha 6 was highly expressed in the first instar larva, pupa and adult, and the expression levels were 3.67, 2.47, 1.38 times that of the second instar larva. The expression level was not significantly different between the susceptible and resistant strains. These results indicate that Foa6 is not involved in resistance to spinosad in F. occidentalis from China and the USA. (C) 2014 Elsevier Inc. All rights reserved.