Attenuation of protein adsorption on static and oscillating magnetostrictive nanowires
Attenuation of protein adsorption on static and oscillating magnetostrictive nanowires
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DOI：
10.1021/nl051117u
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发表时间：
2005-09-01
期刊：
影响因子：
10.8
通讯作者：
Pishko, MV
中科院分区：
文献类型：
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作者：
Ainslie, KM;Sharma, G;Pishko, MV
这里描述的研究调查了纳米线阵列中包含的纳米结构能够减弱医疗器械植入体内时产生的不良宿主反应的假设。这种不利的宿主反应或生物污染在装置和感兴趣的分析物之间产生无血管纤维质传递屏障，如果植入物是传感器，则使植入物失效。许多研究表明，表面化学和结构调节宿主反应。这些发现使我们假设纳米结构表面将显著抑制无血管纤维囊的形成。我们正在研究振荡磁致伸缩纳米线阵列是否可以防止蛋白质吸附。通过将铁磁金属合金电镀到纳米多孔氧化铝模板的孔中，制备了磁致伸缩纳米线。铁磁纳米线通过振荡围绕线的磁场而振荡。使用放射性标记的牛血清白蛋白、酶联免疫吸附测定（ELISA）和其他蛋白质测定来研究纳米线阵列上的蛋白质粘附。这些结果显示静态纳米线的单位表面积的蛋白质吸附减少。比较表面，14 - 30%的蛋白质吸附在平面上吸附在纳米线上。我们的接触角测量表明，蛋白质在纳米线表面上的衰减可能是由于纳米结构表面的亲水性增加相比，相同材料的平坦表面。我们通过将磁致伸缩线放置在38 G 10 Hz的振荡磁场中来振荡磁致伸缩线。振荡纳米线显示出蛋白质粘附的进一步降低，其中在振荡纳米线上测量到静态线上仅7 - 67%的蛋白质。通过改变纳米线振荡的流体的粘度，我们确定蛋白质的分离是剪切应力调制的。我们用右旋糖酐创造了一种高剪切流体，与在基线粘度流体中振荡的纳米线相比，它将振荡纳米线上的蛋白质吸附减少了70%。我们的初步研究强烈地表明，静态纳米线阵列中的结构和通过振荡纳米线阵列产生的剪切将减弱体内的生物污损响应。
The research described here investigates the hypothesis that nanoarchitecture contained in a nanowire array is capable of attenuating the adverse host response generated when medical devices are implanted in the body. This adverse host response, or biofouling, generates an avascular fibrous mass transfer barrier between the device and the analyte of interest, disabling the implant if it is a sensor. Numerous studies have indicated that surface chemistry and architecture modulate the host response. These findings led us to hypothesize that nanostructured surfaces will inhibit the formation of an avascular fibrous capsule significantly. We are investigating whether arrays of oscillating magnetostrictive nanowires can prevent protein adsorption. Magnetostrictive nanowires were fabricated by electroplating a ferromagnetic metal alloy into the pores of a nanoporous alumina template. The ferromagnetic nanowires are made to oscillate by oscillating the magnetic field surrounding the wires. Radiolabeled bovine serum albumin, enzyme-linked immunosorbent assay (ELISA), and other protein assays were used to study protein adhesion on the nanowire arrays. These results display a reduced protein adsorption per surface area of static nanowires. Comparing the surfaces, 14-30% of the protein that absorbed on the flat surface adsorbed on the nanowires. Our contact angle measurements indicate that the attenuation of protein on the nanowire surface might be due to the increased hydrophilicity of the nanostructured surface compared to a flat surface of the same material. We oscillated the magnetostrictive wires by placing them in a 38 G 10 Hz oscillating magnetic field. The oscillating nanowires show a further reduction in protein adhesion where only 7-67% of the protein on the static wires was measured on the oscillating nanowires. By varying the viscosity of the fluid the nanowires are oscillated in, we determined that protein detachment is shear-stress modulated. We created a high shearing fluid with dextran, which reduced protein adsorption on the oscillating nanowires by 70% over nanowires oscillating in baseline viscosity fluid. Our preliminary studies strongly suggest that the architecture in the static nanowire arrays and the shear created by oscillating the nanowire arrays would attenuate the biofouling response in vivo.