Isolation of the REC1 gene controlling recombination in Ustilago maydis.
Isolation of the REC1 gene controlling recombination in Ustilago maydis.
复制标题
分离控制玉米黑粉菌重组的 REC1 基因。
DOI：
10.1016/0378-1119(89)90426-5
复制
发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
Holloman,WK
中科院分区：
文献类型：
--
作者：
Tsukuda,T;Bauchwitz,R;Holloman,WK
先前对黑粉菌重组突变体的研究可能揭示了复杂的表型。该突变体被发现在DNA修复、重组、生长、诱变和减数分裂方面存在缺陷。我们通过使用在复制载体中制作的基因组 DNA 文库补充突变体的表型来克隆 REC1 基因，并通过使用体外构建的突变体等位基因对野生型 (wt)REC1 基因座进行一步基因破坏来提供身份证明。 REC1 基因的转录本被鉴定为损伤诱导型 1.7-kb RNA。紫外线照射后，观察到转录本的水平上升了 10 至 15 倍。在rec1突变体中，1.7-kb转录物的基础水平比wt低十倍。此外，在rec1-1 细胞中还发现了第二个1.1 kb 的转录物，以响应DNA 损伤。
Previous studies on therec1mutant ofUstilago maydisrevealed a complex phenotype. The mutant was found to be defective in DNA repair, recombination, growth, mutagenesis, and meiosis. We cloned theREC1gene by complementing the phenotype of the mutant using a genomic DNA library made in a replicating vector and provided proof of identity by one-step gene disruption of the wild-type (wt)REC1 locus with a mutant allele constructed in vitro. The transcript of theREC1 gene was identified as a damage-inducible 1.7-kb RNA. After UV irradiation, the level of the transcript was observed to rise ten- to 15-fold. In therec1mutant, the basal level of the 1.7-kb transcript was tenfold lower than in wt. In addition, a second transcript of 1.1 kb was noted inrec1-1 cells in response to DNA damage.