ISOLATION AND ULTRASTRUCTURAL CHARACTERIZATION OF THE RESIDUAL NUCLEAR MATRIX IN A PLANT-CELL SYSTEM
ISOLATION AND ULTRASTRUCTURAL CHARACTERIZATION OF THE RESIDUAL NUCLEAR MATRIX IN A PLANT-CELL SYSTEM
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DOI：
10.1007/bf00418244
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发表时间：
1991-02-01
期刊：
影响因子：
1.6
通讯作者：
CEREZUELA, MA
中科院分区：
文献类型：
--
作者：
DELAESPINA, SMD;BARTHELLEMY, I;CEREZUELA, MA
我们分离了洋葱鳞茎增殖分生根细胞的核基质，并通过超微结构和一维SDS-聚丙烯酰胺凝胶电泳对其进行了表征。洋葱根细胞中核基质的一般组织与在相同条件下提取的其他真核生物中发现的相似。有三个主要的形态组成部分：残余孔板复合体，内部基质和残余核。基质具有由5-10 nm原纤维和20-25 nm颗粒组成的原纤维-颗粒结构。残留的蛋白质基质具有复杂的模式，具有12000至70000 Mr的多个多肽条带。主要条带具有62000、56000、35000和27000的Mr。
We isolated the nuclear matrix of proliferative meristematic root cells of Allium cepa bulbs and characterized it ultrastructurally and by one-dimensional SDS-polyacrylamide gel electrophoresis. The general organization of the nuclear matrix in onion root cells is similar to that found in other eukaryotes extracted under the same conditions. There are three main morphological components: the residual pore-lamina complex, the internal matrix and the residual nucleous. The matrix has a fibrillo-granular structure composed of 5-10 nm fibrils and 20-25 nm granules. The residual protein matrix has a complex pattern, with several polypeptide bands of 12000 to 70000 Mr. The major bands have Mrs of 62000, 56000, 35000 and 27000.