Change of dopamine receptor mRNA expression in lymphocyte of schizophrenic patients.
Change of dopamine receptor mRNA expression in lymphocyte of schizophrenic patients.
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DOI：
10.1186/1471-2350-2-3
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发表时间：
2001
影响因子：
--
通讯作者：
Sunwoo I
中科院分区：
文献类型：
--
作者：
Kwak YT;Koo MS;Choi CH;Sunwoo I
虽然已经提出了中枢多巴胺能系统的功能障碍，但由于多巴胺受体的可及性有限，精神分裂症的病因或发病机制仍不确定。本研究的目的是确定易接近的外周淋巴细胞多巴胺受体是否可以作为精神分裂症的外周标志物。3年以上服药精神分裂症患者44例，3个月以上非服药精神分裂症患者28例，drug-naïve精神分裂症患者15例，健康人群31例。采用mRNA序列反转录和定量聚合酶链反应研究外周血淋巴细胞中D3和D5多巴胺受体的表达。比较各组多巴胺受体基因表达情况。无药和drug-naïve患者服用抗精神病药物后，分别于用药后第2周和第8周观察外周血淋巴细胞多巴胺受体的变化。无药物精神分裂症患者外周血淋巴细胞D3多巴胺受体mRNA表达较对照组和有药物精神分裂症患者显著升高，D5多巴胺受体mRNA表达较有药物精神分裂症患者显著升高。服用抗精神病药物后，多巴胺受体mRNA在第2周达到峰值，随后下降，但在第8周高于基线水平。根据用药前多巴胺受体表达情况将无药物组和drug-naïve组患者分为两组，多巴胺受体表达升高组患者精神症状更严重。这些结果表明，分子生物学决定的外周淋巴细胞多巴胺受体具有反应性，外周淋巴细胞多巴胺受体表达升高对精神分裂症亚分可能具有临床意义。
Though the dysfunction of central dopaminergic system has been proposed, the etiology or pathogenesis of schizophrenia is still uncertain partly due to limited accessibility to dopamine receptor. The purpose of this study was to define whether or not the easily accessible dopamine receptors of peripheral lymphocytes can be the peripheral markers of schizophrenia. 44 drug-medicated schizophrenics for more than 3 years, 28 drug-free schizophrenics for more than 3 months, 15 drug-naïve schizophrenic patients, and 31 healthy persons were enrolled. Sequential reverse transcription and quantitative polymerase chain reaction of the mRNA were used to investigate the expression of D3 and D5 dopamine receptors in peripheral lymphocytes. The gene expression of dopamine receptors was compared in each group. After taking antipsychotics in drug-free and drug-naïve patients, the dopamine receptors of peripheral lymphocytes were sequentially studied 2nd week and 8th week after medication. In drug-free schizophrenics, D3 dopamine receptor mRNA expression of peripheral lymphocytes significantly increased compared to that of controls and drug-medicated schizophrenics, and D5 dopamine receptor mRNA expression increased compared to that of drug-medicated schizophrenics. After taking antipsychotics, mRNA of dopamine receptors peaked at 2nd week, after which it decreases but the level was above baseline one at 8th week. Drug-free and drug-naïve patients were divided into two groups according to dopamine receptor expression before medications, and the group of patients with increased dopamine receptor expression had more severe psychiatric symptoms. These results reveal that the molecular biologically-determined dopamine receptors of peripheral lymphocytes are reactive, and that increased expression of dopamine receptor in peripheral lymphocyte has possible clinical significance for subgrouping of schizophrenis.