Increased basigin in bleomycin-induced lung injury
Increased basigin in bleomycin-induced lung injury
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DOI：
10.1165/rcmb.2002-0059oc
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发表时间：
2003-05-01
影响因子：
6.4
通讯作者：
Senior, RM
中科院分区：
文献类型：
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作者：
Betsuyaku, T;Kadomatsu, K;Senior, RM
在发育过程中，Basigin在许多组织中表达，包括肺。它也存在于肿瘤细胞和伤口中，被认为能刺激邻近的成纤维细胞产生基质金属蛋白酶。为了研究basigin是否可能在纤维炎性肺过程中表达，我们制造了博莱霉素诱导的小鼠肺损伤。在气管内注射博莱霉素14天后，我们发现basigin在纤维化区域、肺泡巨噬细胞和细支气管上皮中显著存在，而在未治疗的小鼠中，basigin仅在细支气管上皮中微弱存在。放射免疫沉淀法的Western blots显示，与未处理小鼠肺的ripa不溶性部分相比，博莱霉素处理的肺的ripa不溶性部分显示出增加的basigin。定量逆转录聚合酶链反应显示，博来霉素作用后14 d肺basigin mRNA表达量显著增加，原位杂交显示，细支气管上皮组织中basigin mRNA表达量显著增加。支气管肺泡灌洗液（BALF）的Western blot结果显示，博莱霉素处理后的肺泡灌洗液中不存在各种形式的盆状蛋白。这些结果表明，博莱霉素诱导的肺损伤与细支气管上皮basigin表达增加、纤维化部位basigin沉积和BALF basigin增加有关。因此，基底素可能在弥漫性肺泡损伤中起作用。
Basigin is expressed in many tissues during development, including lung. It is also found on tumor cells and in wounds where it is thought to stimulate adjacent fibroblasts to produce matrix metalloproteinases. To investigate whether basigin might be expressed in fibro-inflammatory lung processes, we generated bleomycin-induced lung injury in mice. At 14 d after intratracheal bleomycin, we found basigin prominently in areas of fibrosis, alveolar macrophages, and bronchiolar epithelium, whereas it was only weakly present in bronchiolar epithelium in untreated mice. Western blots of radioimmunoprecipitation assay RIPA-insoluble fractions of bleomycin-treated lungs showed increased basigin compared with RIPA-insoluble fractions of lung from untreated mice. By quantitative reverse transcriptase-polymerase chain reaction, lung basigin mRNA was significantly increased 14 d after bleomycin, and by in situ hybridization, basigin mRNA was prominent in bronchiolar epithelium. Western blots of bronchoalveolar lavage fluid (BALF) showed various forms of basigin after bleomycin that were not present in BALF from untreated lung. These results demonstrate that bleomycin-induced lung injury is associated with increased basigin expression in bronchiolar epithelium, deposition of basigin in fibrotic sites, and increased basigin in BALF. Accordingly, basigin may play a role in diffuse alveolar injury.