Spatial distributions of guidance molecules regulate chemorepulsion and chemoattraction of growth cones
Spatial distributions of guidance molecules regulate chemorepulsion and chemoattraction of growth cones
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DOI：
10.1523/jneurosci.20-03-01030.2000
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发表时间：
2000-02-01
影响因子：
5.3
通讯作者：
Bolz, J
中科院分区：
文献类型：
--
作者：
Bagnard, D;Thomasset, N;Bolz, J
一般认为趋化性分子的梯度对神经系统的布线是有用的。最近，分泌型III类脑信号蛋白家族的两个成员被认为是皮质轴突的排斥性（Sema3A）和吸引性（Sema3C）引导分子（Bagnard et al.，1998年）。在这里，我们表明，稳定的梯度增加脑信号蛋白浓度引起刻板的反应，从皮质生长锥，独立的绝对浓度和这些梯度的斜率。与此相反，既没有排斥作用的Sema3A，也没有吸引力的Sema3C的影响时，轴突生长朝向降低semaphorin浓度。因此，通过向化分子梯度的生长锥引导是稳健且可重复的，因为它主要独立于梯度的确切尺寸。
It is generally assumed that gradients of chemotropic molecules are instrumental to the wiring of the nervous system. Recently, two members of the secreted class III semaphorin protein family have been implicated as repulsive (Sema3A) and attractive (Sema3C) guidance molecules for cortical axons (Bagnard et al., 1998). Here, we show that stabilized gradients of increasing semaphorin concentrations elicit stereotyped responses from cortical growth cones, independent of the absolute concentration and the slope of these gradients. In contrast, neither repulsive effects of Sema3A nor attractive effects of Sema3C were observed when axons were growing toward decreasing semaphorin concentrations. Thus, growth cone guidance by gradients of chemotropic molecules is robust and reproducible, because it is primarily independent of the exact dimensions of the gradients.