Finite element analysis of microelectrotension of cell membranes
Finite element analysis of microelectrotension of cell membranes
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DOI：
10.1007/s10237-007-0093-y
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发表时间：
2008-10-01
影响因子：
3.5
通讯作者：
Butler, Peter J.
中科院分区：
文献类型：
--
作者：
Bae, Chilman;Butler, Peter J.
电场可以通过基于微量移液器的电极聚焦以在细胞膜上诱导应力，从而导致张力和穿孔。然而，迄今为止，这些膜应力分布尚未被量化。在这项研究中，我们确定膜张力，应力和应变分布在附近的微电极使用有限元分析的多尺度机电模型的吸管，媒体，膜，肌动蛋白皮质，和细胞质。电场力耦合到膜使用麦克斯韦应力张量和膜电压缩理论。结果表明，微管电极提供了一种新的非接触的方法，提供了一个集中和控制的方式直接向膜的生理应力，从而提供了定量的基础micreoelectrotension，膜机械生物学的新技术。
Electric fields can be focused by micropipette-based electrodes to induce stresses on cell membranes leading to tension and poration. To date, however, these membrane stress distributions have not been quantified. In this study, we determine membrane tension, stress, and strain distributions in the vicinity of a microelectrode using finite element analysis of a multiscale electro-mechanical model of pipette, media, membrane, actin cortex, and cytoplasm. Electric field forces are coupled to membranes using the Maxwell stress tensor and membrane electrocompression theory. Results suggest that micropipette electrodes provide a new non-contact method to deliver physiological stresses directly to membranes in a focused and controlled manner, thus providing the quantitative foundation for micreoelectrotension, a new technique for membrane mechanobiology.