A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp pekinensis) Selection
A Genomic Variation Map Provides Insights into the Genetic Basis of Spring Chinese Cabbage (Brassica rapa ssp pekinensis) Selection
复制标题
基因组变异图谱为春白菜（Brassica rapa ssp pekinensis）选择的遗传基础提供了见解
DOI：
10.1016/j.molp.2018.08.006
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发表时间：
2018-11-05
期刊：
影响因子：
27.5
通讯作者：
Zhang, Fenglan
中科院分区：
文献类型：
--
作者：
Su, Tongbing;Wang, Weihong;Zhang, Fenglan
大白菜是东亚国家消费量最大的叶类作物。然而，持续低温诱导的早熟抽薹严重影响了大白菜的产量和品质，限制了大白菜的种植季节和地理分布。40年来，选择冬性强的春大白菜满足市场需求。在这里，我们报告了一个大白菜的基因组变异图谱产生的重测序数据的194个地理多样性的三个生态型。对选择扫描和全基因组模式的深入分析表明，春大白菜是从中国北方山东半岛地区秋大白菜的特定群体中选择出来的。我们确定了23个基因组位点，进行了密集的选择，并进一步证明了基因表达和单倍型分析，纳入精英等位基因的春化不敏感3.1（BrVIN 3.1）和花位点C 1（BrFLC1）是一个决定性的遗传变异来源在选择过程中。此外，我们表明，BrVIN3.1的数量响应冷由于BrVIN3.1启动子的顺式元件的序列变异显着有助于大白菜抽薹时间的变化。总的来说，我们的研究提供了有价值的见解春白菜选择的遗传基础，并将有助于通过分子标记辅助选择，转基因或基因编辑方法培育抗抽薹品种。
Chinese cabbage is the most consumed leafy crop in East Asian countries. However, premature bolting induced by continuous low temperatures severely decreases the yield and quality of the Chinese cabbage, and therefore restricts its planting season and geographic distribution. In the past 40 years, spring Chinese cabbage with strong winterness has been selected to meet the market demand. Here, we report a genome variation map of Chinese cabbage generated from the resequencing data of 194 geographically diverse accessions of three ecotypes. In-depth analyses of the selection sweeps and genome-wide patterns revealed that spring Chinese cabbage was selected from a specific population of autumn Chinese cabbage around the area of Shandong peninsula in northern China. We identified 23 genomic loci that underwent intensive selection, and further demonstrated by gene expression and haplotype analyses that the incorporation of elite alleles of VERNALISATION INSENTIVE 3.1 (BrVIN3.1) and FLOWER LOCUS C 1 (BrFLC1) is a determinant genetic source of variation during selection. Moreover, we showed that the quantitative response of BrVIN3.1 to cold due to the sequence variations in the cis elements of the BrVIN3.1 promoter significantly contributes to bolting-time variation in Chinese cabbage. Collectively, our study provides valuable insights into the genetic basis of spring Chinese cabbage selection and will facilitate the breeding of bolting-resistant varieties by molecular-marker-assisted selection, transgenic or gene editing approaches.