Neuroprotective properties of gallic acid from Sanguisorbae radix on amyloid β protein (25-35)-induced toxicity in cultured rat cortical neurons
Neuroprotective properties of gallic acid from Sanguisorbae radix on amyloid β protein (25-35)-induced toxicity in cultured rat cortical neurons
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DOI：
10.1248/bpb.31.149
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发表时间：
2008-01-01
影响因子：
2
通讯作者：
Seong, Yeon Hee
中科院分区：
文献类型：
--
作者：
Ban, Ju Yeon;Nguyen, Ha Thi Thuy;Seong, Yeon Hee
本课题组前期研究报道了地榆甲醇提取物的化学成分。（蔷薇科）在体外预防A β（25-35）诱导的神经元细胞损伤。本研究采用体外培养的大鼠皮层神经元，观察了地榆没食子酸对A β（25-35）诱导的神经毒性的影响。通过3-[4，5-二甲基噻唑-2-基]-2，5-二苯基-溴化四唑（MTT）测定和Hoechst 33342染色评估，没食子酸（0.1，1 μ M）显示对A β（2,5 -35）（10 μ M）诱导的凋亡性神经元死亡的浓度依赖性抑制。没食子酸预处理抑制10 μ M A β（25-35）诱导的胞质Ca 2+浓度（[Ca 2 +]（c））升高和活性氧（ROS）的产生，这通过荧光染料测量。没食子酸还抑制由10 μ M A β（25-35）诱导的谷氨酸释放到培养基中，这通过HPLC测量。这些结果表明，没食子酸通过干扰[Ca 2 +]（c）的增加，然后通过抑制谷氨酸的释放和ROS的产生来防止A β（25-35）诱导的凋亡性神经元死亡，并且没食子酸的这些作用可能部分地与地榆的神经保护作用有关。
Our previous studies reported that methanol extract of Sanguisorbae radix from Sanguisorba officinails L. (Rosaceae) prevented neuronal cell damage induced by A beta (25-35) in vitro. The present study was carried out to investigate the effect of gallic acid isolated from Sanguisorbae radix on A beta (25-35)-induced neurotoxicity using cultured rat cortical neurons. Gallic acid (0.1, 1 mu M) showed a concentration-dependent inhibition on A beta (25-35) (10 mu M)-induced apoptotic neuronal death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. Pretreatment of gallic acid inhibited 10 mu M A beta (25-35)-induced elevation of cytosolic Ca2+ concentration ([Ca2+](c)) and generation of reactive oxygen species (ROS), which were measured by fluorescent dyes. Gallic acid also inhibited glutamate release into medium induced by 10 mu M A beta (25-35), which was measured by HPLC. These results suggest that gallic acid prevents A beta (25-35)induced apoptotic neuronal death by interfering with the increase of [Ca2+](c), and then by inhibiting glutamate release and generation of ROS, and that these effects of gallic acid may be partly associated with the neuroprotective effect of Sanguisorbae radix.