Biochemical analysis of wound fluid from nonhealing and healing chronic leg ulcers
Biochemical analysis of wound fluid from nonhealing and healing chronic leg ulcers
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DOI：
10.1046/j.1524-475x.1996.40211.x
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发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Stacey, Michael C.
中科院分区：
文献类型：
--
作者：
Trengove, Naomi J.;Langton, Simon R.;Stacey, Michael C.
本研究的目的是确定慢性伤口液的生化组成，并将这些值与血清的生化组成进行比较，从而评估伤口液是否代表伤口的细胞外环境。对8例处于未愈合期和愈合期的慢性腿部溃疡患者收集伤口液和血液样本。对伤口液和血清样本进行筛选，以进行一般生化分析，包括电解质、乳酸、葡萄糖和蛋白质分析。伤口液和血清样本中的电解质水平基本相同。与配对的血清样本相比，伤口液中的乳酸和乳酸脱氢酶水平显著升高，葡萄糖和碳酸氢盐水平显著降低。伤口液中的白蛋白和总蛋白水平平均为血清水平的一半。在这个8例患者的小样本中，从慢性腿部溃疡收集的伤口液是一种渗出物，具有细胞外液中预期的生化成分。此外，在愈合过程中，伤口液中碳酸氢盐和葡萄糖水平升高，c反应蛋白水平降低，但血清中保持不变。这些结果提示缺氧状态的变化和愈合伤口的炎症过程。
The purpose of this study was to determine the biochemical composition of fluid taken from chronic wounds, to compare these values with that of serum, and therefore to assess whether the wound fluid is representative of the extracellular environment of the wound. Paired wound fluid and blood samples were collected from eight patients with chronic leg ulcers in a nonhealing and healing phase. Wound fluid and serum samples were screened for a profile of general biochemical analyses, including electrolytes, lactate, glucose, and protein analyses. Electrolyte levels were essentially identical in wound fluid and serum samples. Lactate and lactate dehydrogenase levels were significantly greater and glucose and bicarbonate levels were significantly lower in wound fluid when compared with the paired serum samples. Albumin and total protein levels in wound fluid were on average half those of serum levels, In this small sample of eight patients, wound fluid collected from chronic leg ulcers is an exudate with the biochemical composition expected in extracellular fluid. In addition, bicarbonate and glucose levels increase and C-reactive protein levels decrease in wound fluid, but remain unchanged in serum, during healing. These results suggest changes in the state of hypoxia and the inflammatory process in the healing wound.