Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype
Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype
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猪轮状病毒 YM（一种可能的新轮状病毒血清型）的分子和抗原特征
DOI：
10.1128/jvi.62.11.4331-4336.1988
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发表时间：
1988
影响因子：
5.4
通讯作者：
C. Arias
中科院分区：
文献类型：
--
作者：
A. M. Ruiz;Imelda V. Lopez;S. López;R. Espejo;C. Arias
1983年，我们分离出一种猪轮状病毒（毒株YM），根据其特征电泳型的频率判断，该病毒在墨西哥的几个地区流行。通过减焦中和试验，轮状病毒YM与血清型1 （Wa）、2 （S2）、3 （SA11）、4 （ST3）、5 （OSU）和6 （NCDV）的轮状病毒原型株有明显区别。不同轮状病毒株的抗血清与轮状病毒YM孵育时，观察到轻微的单向交叉中和（1 - 5%）。此外，对血清型1、2、3和5具有特异性的中和单克隆抗体不能中和YM病毒。酶联免疫吸附试验确定病毒亚群为I。为了在分子水平上表征病毒的血清型特异性糖蛋白，我们克隆了VP7的编码基因并对其进行了测序。将推断的氨基酸序列与已报道的六种不同血清型的人和动物轮状病毒株的同源序列进行比较，进一步支持了YM VP7蛋白的独特免疫学特性。
In 1983, we isolated a porcine rotavirus (strain YM) that was prevalent in several regions of Mexico, as judged by the frequency of its characteristic electropherotype. By a focus reduction neutralization test, rotavirus YM was clearly distinguished from prototype rotavirus strains belonging to serotypes 1 (Wa), 2 (S2), 3 (SA11), 4 (ST3), 5 (OSU), and 6 (NCDV). Minor, one-way cross-neutralization (1 to 5%) was observed when antisera to the various rotavirus strains were incubated with rotavirus YM. In addition, the YM virus was not neutralized by neutralizing monoclonal antibodies with specificity to serotypes 1, 2, 3, and 5. The subgroup of the virus was determined to be I by enzyme-linked immunosorbent assay. To characterize the serotype-specific glycoprotein of the virus at the molecular level, we cloned and sequenced the gene coding for VP7. Comparison of the deduced amino acid sequence with reported homologous sequences from human and animal rotavirus strains belonging to six different serotypes further supported the distinct immunological identity of the YM VP7 protein.
影响因子：
3.7
作者：
Chan,WK;Penaranda,ME;Crawford,SE;Estes,MK
通讯作者：
Estes,MK