Multi-Layered Plasma-Polymerized Chips for SPR-Based Detection
Multi-Layered Plasma-Polymerized Chips for SPR-Based Detection
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DOI：
10.1021/am201061k
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发表时间：
2011-12-01
影响因子：
9.5
通讯作者：
Williams, David E.
中科院分区：
文献类型：
--
作者：
Gandhiraman, Ram P.;Nam Cao Hoai Le;Williams, David E.
贵金属的表面功能化在基于表面等离子体共振的生物分子检测系统中是至关重要的，因为界面涂层必须在增强最佳负载的同时保持固定化生物分子的活性。我们在这里提出了一个一步，室温，高速，气相等离子体聚合工艺，用于功能化金衬底，使用硅氧烷作为粘合层，丙烯酸作为功能层。比较了硅氧烷和硫醇基涂层的粘附性能和后续功能化的界面层性能。硅氧烷和丙烯酸的原位顺序沉积导致羧基功能表面含量比含有硫醇前体沉积的膜增加7倍。通过二次离子质谱研究证实，在等离子体的应用下，由于离子诱导的表面涂层混合而获得的层组成的分级。在Biacore 3000 SPR仪器中，利用表面等离子体增强椭偏仪在金/玻璃底物上验证了DNA杂交分析，并在等离子体功能化的金/塑料底物上验证了该涂层对蛋白质免疫分析的适用性。
The surface functionalization of a noble metal is crucial in a surface plasmon resonance-based biomolecular detection system because the interfacial coating must retain the activity of immobilized biomolecules while enhancing the optimal loading. We present here a one-step, room-temperature, high-speed, gas-phase plasma polymerization process for functionalizing gold substrates using siloxane as an adhesion layer and acrylic acid as a functional layer. Siloxane- and thiol-based coatings were compared for their performance as adhesion and the interfacial layer for subsequent functionalization. An in situ sequential deposition of siloxane and acrylic acid resulted in a 7-fold increase in carboxylic functionality surfacial content compared to films deposited with thiol-containing precursors. Grading of the layer composition achieved as a consequence of ion-induced mixing on the surface coating under the application of the plasma is confirmed through secondary ion mass spectroscopic studies. DNA hybridization assays were demonstrated on gold/glass substrates using surface plasmon enhanced ellipsometry and the applicability of this coating for protein immunoassays were demonstrated with plasma functionalized gold/plastic substrates in Biacore 3000 SPR instrument.