Extracellular matrix proteins secreted from both the endometrium and the embryo are required for attachment: A study using a co-culture model of rat blastocysts and Ishikawa cells
Extracellular matrix proteins secreted from both the endometrium and the embryo are required for attachment: A study using a co-culture model of rat blastocysts and Ishikawa cells
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DOI：
10.1002/jmor.20076
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发表时间：
2013-01-01
影响因子：
1.5
通讯作者：
Day, Margot L.
中科院分区：
文献类型：
--
作者：
Kaneko, Yui;Murphy, Christopher R.;Day, Margot L.
整合素在着床过程中在母胎界面以高度调节的方式表达。然而，细胞外基质（ECM）配体在整合素介导的胚胎附着到子宫内膜的意义还没有完全理解。因此，在植入时研究了纤维连接蛋白在大鼠子宫和胚泡中的分布。纤连蛋白在子宫腔上皮细胞中不存在，但在滋养层细胞和内细胞团中强烈表达，这表明胚泡分泌的纤连蛋白可能是母胎界面表达的整合素的可能桥接配体。用Arg-Gly-Asp（RGD）肽封闭整合素上的RGD识别位点，并检测其对大鼠胚泡与石川细胞粘附的影响。当胚泡或石川细胞与RGD阻断肽预孵育时，胚泡附着显著减少。因此，胚胎与子宫内膜的成功附着需要子宫内膜和胚泡上的整合素与ECM配体（如纤连蛋白）的RGD序列相互作用。用RGD肽预处理囊胚和石川细胞也抑制囊胚附着，但不完全，表明不含RGD序列的ECM桥接配体也参与胚胎附着。J. Morphol. 2013. (c)2012 Wiley Periodicals，Inc.
Integrins are expressed in a highly regulated manner at the maternal-fetal interface during implantation. However, the significance of extracellular matrix (ECM) ligands during the integrin-mediated embryo attachment to the endometrium is not fully understood. Thus, the distribution of fibronectin in the rat uterus and blastocyst was studied at the time of implantation. Fibronectin was absent in the uterine luminal epithelial cells but was intensely expressed in the trophoblast cells and the inner cell mass suggesting that fibronectin secreted from the blastocyst may be a possible bridging ligand for the integrins expressed at the maternal-fetal interface. An Arg-Gly-Asp (RGD) peptide was used to block the RGD recognition sites on integrins, and the effect on rat blastocyst attachment to Ishikawa cells was examined. There was a significant reduction in blastocyst attachment when either the blastocysts or the Ishikawa cells were pre-incubated with the RGD-blocking peptide. Thus, successful attachment of the embryo to the endometrium requires the interaction of integrins on both the endometrium and the blastocyst with the RGD sequence of ECM ligands, such as fibronectin. Pre-treatment of both blastocysts and Ishikawa cells with the RGD peptide also inhibited blastocyst attachment, but not completely, suggesting that ECM bridging ligands that do not contain the RGD sequence are also involved in embryo attachment. J. Morphol. 2013. (c) 2012 Wiley Periodicals, Inc.