Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"
Production and characterization of Brassica napus-Raphanus sativus monosomic addition lines mediated by the synthetic amphidiploid "Raphanobrassica"
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DOI：
10.1270/jsbbs.59.109
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发表时间：
2009-06-01
期刊：
影响因子：
2.4
通讯作者：
Matsuzawa, Yasuo
中科院分区：
文献类型：
--
作者：
Akaba, Michiko;Kaneko, Yukio;Matsuzawa, Yasuo
为了培育甘蓝型油菜-萝卜单体附加系(MAL)，进行了两种合成双倍体(RRAA和RRCC)和甘蓝型油菜(AACC)之间的杂交。通过染色体加倍从每个F杂交产生两个同种异体八倍体（RRAAAACC和AACCRRCC）。通过与甘蓝型油菜的连续回交，在BC2代中首次获得了MAL植物，并且通过基因组原位杂交(GISH)鉴定了来自R. sativus的染色体。 MAL 植物中 R 基因组的 9 条染色体通过每个染色体特异性 RAPD 标记进行了清晰的分类。结果，异质（萝卜细胞质）B. napus-R。 BC3、BC4代获得了具有8种类型(a-i，h型除外)的苜蓿MAL和具有完整9种类型(a-i)的自质(油菜胞质)MAL。这些异质和自质MAL植物在形态、生理和细胞遗传学特征上表现出差异。从有利性状调查结果表明，a型具有异质系雄性不育恢复育性基因，g型具有控制白色花瓣的基因。这两个MAL是探索位于萝卜每条染色体上的农艺性状以及促进有希望的萝卜基因渗入甘蓝型油菜的有用材料。
In order to breed Brassica napus-Raphanus sativus monosomic addition lines (MALs), hybridizations between two synthetic amphidiplold (RRAA and RRCC) and B. napus (AACC) were performed. Two allooctoploids (RRAAAACC and AACCRRCC) were produced from each F, hybrid by chromosome doubling. From successive backcrosses to B. napus, MAL plants were first obtained in BC2, generation and the R. sativus-derived chromosome was identified by genomic in situ hybridization (GISH). The nine chromosomes of R-genome in the MAL plants were clearly classified by each chromosome-specific RAPD markers. As a result, alloplasmic (radish cytoplasm) B. napus-R. sativus MAL having 8 types (a-i, except for h-type) and autoplasmic (rape cytoplasm) MAL with complete 9 types (a-i) were obtained in BC3 and BC4 generations. These alloplasmic and autoplasmic MAL plants were showed differences in their morphological, physiological and cytogenetical characters. From the survey of favorable traits, it was suggested that the a-type had fertility restoring gene(s) for male sterility in alloplasmic line and the g-type had a gene controlling white color petal. These two MALs are useful materials for exploring agronomic traits located on each chromosome of radish and for promoting the introgression of promising radish gene(s) to B. napus.