Evidence for two cDNA clones encoding human GM2-activator protein.

Evidence for two cDNA clones encoding human GM2-activator protein.
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两个编码人类 GM2 激活蛋白的 cDNA 克隆的证据。

DOI:
10.1042/bj2820807
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发表时间:
1992
期刊:
The Biochemical journal
影响因子:
--
通讯作者:
Lockyer,JM
Lockyer,JM
中科院分区:
--
文献类型:
--
作者:
Nagarajan,S;Chen,HC;Li,SC;Li,YT;Lockyer,JM

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从人胎盘λ gt 11文库中分离到两个编码GM 2激活剂的cDNA,pGM 2A(648 bp)和GAP(1093 bp)。pGM 2A的DNA序列从1到302与GAP几乎相同,但在303-648之间存在差异。PCR用于证明胎盘RNA中存在两种GM 2激活剂。两种cDNA均与约2.3 kb的mRNA杂交,并与基因组Southern印迹上相同的单一条带杂交。
Two cDNAs encoding GM2 activator, pGM2A (648 bp) and GAP (1093 bp), were isolated from human placenta lambda gt11 libraries. The DNA sequence of pGM2A from 1 to 302 was almost identical with GAP, but diverged from 303-648. PCR was used to demonstrate the presence of both species of GM2 activator in placental RNA. Both cDNAs hybridized to mRNAs of approximately 2.3 kb and to identical single bands on genomic Southern blots.
DOI: --
发表时间: 1985
期刊:
影响因子: --
作者:
J. Lucy
通讯作者: J. Lucy